DNAJC9 PN Consistency Notes
- Generated: 2026-06-18
- Project: PROTEOSTASIS
- Scope: PN consistency rereview against local AIGR review and available deep-research artifacts
- UniProt: Q8WXX5
- AIGR review status: COMPLETE
- Review batch: proteostasis-batch-2026-06-07b
- Batch change status: added
Source Files Checked
Deep Research Files
- No
*-deep-research*.md file found in this gene directory.
AIGR Review Snapshot
- Description: DNAJC9 (also called HDJC9 or DnaJ protein SB73) is a type C DnaJ/Hsp40 (DNAJC) co-chaperone with an N-terminal J domain and a C-terminal histone-binding domain. It has a dual role as a histone H3-H4 chaperone and an HSP70 heat-shock co-chaperone. As a histone chaperone it forms a co-chaperone complex with MCM2 and histone H3-H4 heterodimers, binds H3 variants (H3.1, H3.2, H3.3) and H4, and integrates HSP70-mediated ATP-driven protein folding into the histone supply chain during replication- and transcription-coupled nucleosome assembly, helping to resolve aberrant histone-folding intermediates and assemble histones into nucleosomes. Its J domain recruits and stimulates the ATPase activity of HSP70-type chaperones (HSPA1A, HSPA1B, HSPA8). DNAJC9 is predominantly nuclear under normal conditions and translocates to the cytoplasm and plasma membrane after heat shock via a non-classical, lipid-dependent pathway; its expression is induced by heat shock, LPS, PMA and TNF.
- Existing/core annotation action counts: ACCEPT: 12; KEEP_AS_NON_CORE: 12; MODIFY: 1
PN Consistency Summary
- Consistency: Strong. Notes, review YAML, and PN all agree DNAJC9 is a J-domain HSP70 co-chaperone (binds HSPA1A/1B/8 via J domain, stimulates HSP70 ATPase). No contradictions. The review additionally develops the dual histone-H3-H4 chaperone role (MCM2 complex; PMID:33857403) that the single PN row does not capture, but this is enrichment, not conflict.
- PN story / NEW pressure: PN asserts only Hsp70-binding, which is captured: GOA/review already carry GO:0031072 (heat shock protein binding, parent of GO:0030544) plus GO:0032781, GO:0051087, GO:0101031. The projected GO:0030544 (verified real, child of GO:0031072) is a defensible refinement (
goa_status=more_specific_than_existing_goa is accurate — GOA has the parent GO:0031072). No NEW term needed; the histone-chaperone axis (GO:0042393, GO:0006334) is the gene's distinctive biology and is already fully annotated in the review.
- Evidence alignment: PN row carries no reference titles; review anchors on PMID:17182002 (HSP70 co-chaperone, verified) and PMID:33857403 (histone chaperone, verified). No divergence — review evidence is a superset.
- Verdict: Consistent; PN GO:0030544 already captured (as parent in GOA) and defensible. No edits required.
Full Consistency Review
- UniProt: Q8WXX5 · batch: proteostasis-batch-2026-06-07b · review status: COMPLETE
- PN placement:
Cytonuclear proteostasis|Chaperone|HSP70 system|J-domain containing HSP70 cochaperone ; PN-node mapping: type=mapped, scope=ok_for_propagation_to_go, GO:0030544 Hsp70 protein binding (group/class/branch = no_mapping)
- Consistency: Strong. Notes, review YAML, and PN all agree DNAJC9 is a J-domain HSP70 co-chaperone (binds HSPA1A/1B/8 via J domain, stimulates HSP70 ATPase). No contradictions. The review additionally develops the dual histone-H3-H4 chaperone role (MCM2 complex; PMID:33857403) that the single PN row does not capture, but this is enrichment, not conflict.
- PN story / NEW pressure: PN asserts only Hsp70-binding, which is captured: GOA/review already carry GO:0031072 (heat shock protein binding, parent of GO:0030544) plus GO:0032781, GO:0051087, GO:0101031. The projected GO:0030544 (verified real, child of GO:0031072) is a defensible refinement (
goa_status=more_specific_than_existing_goa is accurate — GOA has the parent GO:0031072). No NEW term needed; the histone-chaperone axis (GO:0042393, GO:0006334) is the gene's distinctive biology and is already fully annotated in the review.
- Mapping strategy: This gene does not require changing the node. GO:0030544 is appropriately narrower than the review's broad coverage and is defensible for the J-domain-cochaperone type. The PN row legitimately under-describes DNAJC9 (ignores histone role), but that is by-design for a type-level mapping.
- Evidence alignment: PN row carries no reference titles; review anchors on PMID:17182002 (HSP70 co-chaperone, verified) and PMID:33857403 (histone chaperone, verified). No divergence — review evidence is a superset.
- Verdict: Consistent; PN GO:0030544 already captured (as parent in GOA) and defensible. No edits required.
PN Dossier Context
- review_batch: proteostasis-batch-2026-06-07b
- review_yaml: genes/human/DNAJC9/DNAJC9-ai-review.yaml
- PN workbook rows: 1
PN row 1: Cytonuclear proteostasis | Chaperone | HSP70 system | J-domain containing HSP70 cochaperone
- UniProt: Q8WXX5
- In branches: CY
- PN-node mapping records (path + ancestors):
- [type] Cytonuclear proteostasis|Chaperone|HSP70 system|J-domain containing HSP70 cochaperone
status=mapped scope=ok_for_propagation_to_go GO=[GO:0030544 Hsp70 protein binding]
rationale: In the PN hierarchy, this type denotes J-domain cochaperones assigned to the HSP70 system. Their shared mechanistic role is direct interaction with HSP70-family chaperones, making Hsp70 protein binding the most defensible GO target in the current cache.
- [group] Cytonuclear proteostasis|Chaperone|HSP70 system
status=no_mapping scope= GO=[]
rationale: Reviewed as a broad PN category rather than a specific GO class. The member genes span multiple activities, complexes, or contexts, so propagation from this node would overstate the shared biology; use narrower child or gene-level curations.
- [class] Cytonuclear proteostasis|Chaperone
status=no_mapping scope= GO=[]
rationale: Reviewed as a broad PN category rather than a specific GO class. The member genes span multiple activities, complexes, or contexts, so propagation from this node would overstate the shared biology; use narrower child or gene-level curations.
- [branch] Cytonuclear proteostasis
status=no_mapping scope= GO=[]
rationale: Reviewed as a top-level PN branch. This is a systems/taxonomy umbrella, not a direct GO assertion; narrower child curations carry any propagating GO mappings.
Projected GO annotations (1)
- GO:0030544 Hsp70 protein binding | scope=ok_for_propagation_to_go | goa_status=more_specific_than_existing_goa | from=Cytonuclear proteostasis|Chaperone|HSP70 system|J-domain containing HSP70 cochaperone
Note
This file is generated from the current PROTEOSTASIS phase-1 dossier and local gene-review artifacts. Edit the source review, PN mapping, or dossier rather than this generated note when correcting the underlying curation.