Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
The combined structural and kinetic characterization of a bacterial nitronate monooxygenase from Pseudomonas aeruginosa PAO1 establishes NMO class I and II.
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Purified PA4202 oxidises propionate 3-nitronate and other alkyl nitronates but not nitroalkanes, with P3N the best substrate at pH 7.5.
"The enzyme is active on P3N and other alkyl nitronates, but cannot oxidize nitroalkanes."
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The enzyme binds FMN tightly and non-covalently and its 1.44 A structure shows a TIM barrel fold; four shared motifs define class I NMO.
"Absorption spectroscopy, mass spectrometry, and x-ray crystallography demonstrate a tightly, non-covalently bound FMN in the active site of the enzyme."
Pseudomonas aeruginosa LysR PA4203 regulator NmoR acts as a repressor of the PA4202 nmoA gene, encoding a nitronate monooxygenase.
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Deletion of nmoA reduces tolerance of 3-nitropropionate and abolishes the dose-dependent nitrite release seen in wild type, supporting a detoxification role in vivo.
"Finally, deletion of the nmoA gene resulted in an increased sensitivity of the cells to 3-nitropropionic acid (3-NPA), confirming the role of the nitronate monooxygenase protein in the detoxification of nitronate."
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nmoA is directly repressed by the LysR-family regulator NmoR (PA4203).
"Here we show that PA4203 ( nmoR ) represses its own transcription and the expression of nmoA."
Growth of bacteria on 3-nitropropionic acid as a sole source of carbon, nitrogen, and energy.
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Bacteria grow on 3-nitropropionate and P3N as sole carbon, nitrogen and energy source; the denitration step is catalysed by a purified monooxygenase whose gene belongs to the group previously annotated as 2-nitropropane dioxygenases.
"Stoichiometry of the reaction indicates that the enzyme is a monooxygenase."
Kinetic Investigation of a Presumed Nitronate Monooxygenase from Pseudomonas aeruginosa PAO1 Establishes a New Class of NAD(P)H:Quinone Reductases.
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Purified PA0660, a leaf of the same PAINT node as NmoA, has no nitronate monooxygenase activity and is instead an NAD(P)H:quinone reductase.
"Despite its annotation, PA0660 did not exhibit nitronate monooxygenase activity."