PANTHER Family Review - Laminin/Netrin Extracellular Matrix (PTHR10574)
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NTN1/NTN3 have erroneous IBA annotations from PANTHER node PTN000180816 which incorrectly grouped netrins with POU-domain TFs
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PTHR10574 proteins are secreted ECM/guidance proteins with no DNA-binding domains
Annotation inferences using phylogenetic trees
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Electronic Gene Ontology annotations created by ARBA machine learning models
A Floor-Plate Extracellular Protein-Protein Interaction Screen Identifies Draxin as a Secreted Netrin-1 Antagonist.
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Demonstrates protein-protein interaction between netrins and DRAXIN
"The assembled floor-plate network contains 47 interactions including the hitherto-not-reported interaction between Netrin-1 and Draxin."
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DRAXIN competes with netrin receptors for binding to Netrin-1
"demonstrated that Draxin competes with Netrin receptors for binding to Netrin-1"
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NTN3 interaction with DRAXIN confirmed by IntAct (2 experiments)
"The assembled floor-plate network contains 47 interactions including the hitherto-not-reported interaction between Netrin-1 and Draxin."
The NTN2L gene encoding a novel human netrin maps to the autosomal dominant polycystic kidney disease region on chromosome 16p13.3.
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First description of human NTN3 (originally called NTN2L)
"We describe the characterization of a novel human netrin-2-like gene, designated NTN2L, and its transcript."
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Netrins are chemotropic factors central to axon guidance
"The netrins define a family of chemotropic factors that have been shown to play a central role in axon guidance."
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NTN3 has restricted expression pattern, detected in spinal cord by RT-PCR
"The NTN2L gene has a restricted pattern of expression; its transcript is undetectable by Northern analysis in all tissues examined, but can be recovered from spinal cord RNA by RT-PCR."
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Predicted 580-amino-acid protein with homology to chicken, Drosophila netrins and C. elegans UNC-6
"The NTN2L gene is predicted to encode a 580-amino-acid protein having homology to the chicken and Drosophila netrins and to Caenorhabditis elegans UNC-6."
Falcon deep research report for human NTN3
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NTN3 encodes secreted Netrin-3, a laminin-like extracellular guidance cue that binds canonical netrin receptors including DCC, NEO1, and UNC5-family receptors.
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Binding data summarized in the report support NTN3 as a netrin receptor ligand, with comparatively strong binding to UNC5B and UNC5C in available datasets.
Netrin-3, a mouse homolog of human NTN2L, is highly expressed in sensory ganglia and shows differential binding to netrin receptors.