TreeGrafter-generated GO annotations

file:PSEPK/nicD/nicD-uniprot.txt

UniProtKB reviewed entry for nicD

Curated reaction annotation is tied to the original KT2440 primary study.

"Reaction=N-formylmaleamate + H2O = maleamate + formate + H(+);"

Deciphering the genetic determinants for aerobic nicotinic acid degradation: the nic cluster from Pseudomonas putida KT2440.

Recombinant NicD extracts generate maleamate and formate, and mutagenesis supports S101, D125 and H245; these are per-enzyme results, not just ensemble reconstruction.

"Further conversion of N-formylmaleamic acid to formic and maleamic acid is catalyzed by the NicD protein"

PMID:22530095

Genomic analysis of Pseudomonas putida: genes in a genome island are crucial for nicotine degradation.

Purified S16 Nfo, Ami and Iso execute the same downstream reactions but do not establish a complete nicotinate route. S16 resting cells failed to degrade nicotinate in the reported test.

"Attempts to degrade nicotinic acid by resting cells of strain S16 did not result in any degradation after 24 h of incubation"

file:interpro/panther/PTHR43798/PTHR43798-paint.tsv

PANTHER PAINT annotations for PTHR43798

file:PSEPK/nicD/nicD-deep-research-falcon.md

Falcon deep research report for nicD