Gene Ontology annotation based on Enzyme Commission mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
The genome sequence of the anaerobic, sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough
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Genome sequence and gene annotation for D. vulgaris Hildenborough including aprB (DVU_0846)
"The 3,570,858 base pair (bp) genome sequence reveals a network of novel c-type cytochromes"
Phylogeny of the alpha and beta subunits of the dissimilatory adenosine-5′-phosphosulfate (APS) reductase from sulfate-reducing prokaryotes – origin and evolution of the dissimilatory sulfate-reduction pathway
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AprAB heterodimer structure with AprA containing FAD and AprB containing two [4Fe-4S] clusters
"reductases are highly conserved among SRP and SOB and form heterodimers with one alpha subunit (75–80 kDa, one FAD) and one beta subunit (18–23 kDa, two [4Fe–4S] centres) which are encoded by the aprBA gene loci"
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APS reductase is the second of the three key enzymes of dissimilatory sulfate reduction, converting APS to AMP and sulfite
"After the activation of the chemically inert sulfate to adenosine-5 9-phosphosulfate (APS) by ATP sulfurylase (Sat), the second enzyme, APS reductase (Apr), converts APS to AMP and sulfite, which is finally reduced to sulfide by the activity of the sulfite reductase (Dsr)"
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Gene clustering of aprBA with qmoABC in SRP genomes
"The sat/aprBA/qmoABC gene organization of the available genomes reflected the phylogenetic divergence of the investigated SRP species into the four major AprBA lineages"
Homology Modeling of Dissimilatory APS Reductases (AprBA) of Sulfur-Oxidizing and Sulfate-Reducing Prokaryotes
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AprB has ferredoxin-like architecture with two [4Fe-4S] clusters
"Its beta-subunit can be subdivided in three segments comprising a bacterial ferredoxin-like segment that envelopes both [4Fe-4S] clusters (amino acids B1-B68)"
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Electron flow from proximal to distal cluster then to AprA FAD
"The two electrons required for the reduction of APS were postulated to be transferred one by one over 30 A˚ via [4Fe-4S] cluster II at the surface of the protein, cluster I and Trp-B48 to the isoalloxazine ring of the buried FAD [18,23,24]"
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Conserved structural features across SRP lineages
"The almost identical protein matrices surrounding both [4Fe-4S] clusters, the FAD cofactor, the active site channel and center within the AprB/A models of SRP and SOB point to a highly similar catalytic process of APS reduction/sulfite oxidation"
The membrane QmoABC complex interacts directly with the dissimilatory adenosine 5′-phosphosulfate reductase in sulfate reducing bacteria
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Direct physical interaction between QmoABC and AprAB with KD approximately 90 nM
"This showed that the QmoABC–AprAB complex has a strong steady-state affinity (K D = 90 ± 3 nM), but has a transient character due to a fast dissociation rate."
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APS reductase (AprAB) is a soluble terminal reductase, not membrane integral
"The two terminal reductases, APS reductase (AprAB) and dissimilatory sulfite reductase (DsrAB), are soluble and thus not directly involved in membrane-linked electron transport."
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QmoA is the principal subunit mediating contact with AprAB
"Far-Western blot identified QmoA as the Qmo subunit most involved in the interaction."
Deep research summary for aprB (Q72DT3) in D. vulgaris Hildenborough