Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Receptor-mediated activation of heterotrimeric G-proteins in living cells.
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Occupied cAR1, whether or not phosphorylated, catalyzes the heterotrimeric G-protein cycle, with rapid dissociation/reassociation on cAMP addition/removal.
"occupied receptors, whether or not they are phosphorylated, catalyze the G-protein cycle"
Chemoattractant signaling in dictyostelium discoideum.
A rapid and efficient method to generate multiple gene disruptions in Dictyostelium discoideum using a single selectable marker and the Cre-loxP system.
Constitutively active G protein-coupled receptor mutants block dictyostelium development.
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cAR1 is a GPCR for cAMP required for multicellular development; its pathway activation is transient due to adaptation.
"The activation of multiple pathways by cAR1 is transient because of poorly defined adaptation mechanisms"
Overexpression of the cAMP receptor 1 in growing Dictyostelium cells.
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cAR1 binds cAMP with nanomolar affinity (two affinity states, Kd ~30 and 300 nM).
"two affinity states with Kd's of"
Regulation of G protein-coupled cAMP receptor activation by a hydrophobic residue in transmembrane helix 3.
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cAR1 is essential for multicellular development; Ile-104 substitutions elevate cAMP affinity and can lock the receptor active.
"cAR1, a G protein-coupled cAMP receptor, is essential for multicellular development of Dictyostelium"
Developmental commitment in Dictyostelium discoideum.
A spatially restricted increase in receptor mobility is involved in directional sensing during Dictyostelium discoideum chemotaxis.
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Agonist stimulation increases the mobile fraction of cAMP receptor at the leading edge, contributing to directional sensing.
"an agonist-induced increase in the mobile fraction of cAMP-receptor at the leading edge of chemotacting Dictyostelium discoideum cells"
Gene targeting of the aggregation stage cAMP receptor cAR1 in Dictyostelium.
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cAR1-null mutants fail to bind or sense cAMP and arrest in early development, proving cAR1 controls development.
"fail to bind or sense cAMP and arrest in early development"
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cAR1-mediated signal transduction controls Dictyostelium development.
"cAR1-mediated signal transduction controls the development of Dictyostelium"
Chemoattractant-mediated Rap1 activation requires GPCR/G proteins.
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Chemoattractant-induced Rap1 activation requires cAR1/cAR3 and Galpha2.
"The rapid Rap1 activation upon cAMP chemoattractant stimulation was absent in cells lacking chemoattractant cAMP receptors cAR1/cAR3"
Quantification of GPCR internalization by single-molecule microscopy in living cells.
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Persistent agonist stimulation increases the cytosolic fraction of cAR1 via phosphorylation-dependent internalization.
"the fraction of cytosolic receptors increases after persistent agonist stimulation"
The green tea catechin epigallocatechin gallate (EGCG) blocks cell motility, chemotaxis and development in Dictyostelium discoideum.
Phosphorylation of chemoattractant receptors regulates chemotaxis, actin reorganization and signal relay.
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cAR1 phosphorylation is required for adenylyl cyclase adaptation and oscillatory cAMP wave production.
"receptor phosphorylation is required to regulate adenylyl cyclase adaptation, long-range oscillatory cAMP wave production and cytoskeletal actin response"
Arrestins function in cAR1 GPCR-mediated signaling and cAR1 internalization in the development of Dictyostelium discoideum.
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Activation of cAR1 recruits arrestin AdcC to the membrane and links the receptor to periodic ERK2 activation and internalization.
"activation of cAR1 promotes the transient membrane recruitment of AdcC and interaction with cAR1"
The Dictyostelium prestalk inducer differentiation-inducing factor-1 (DIF-1) triggers unexpectedly complex global phosphorylation changes.
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DIF-1 rapidly dephosphorylates the cAMP receptor, reflecting DIF-1/cAMP signaling antagonism.
"the rapid dephosphorylation of the cAMP receptor that we observe in response to DIF-1"
Response of Dictyostelium discoideum to UV-C and involvement of poly (ADP-ribose) polymerase.
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carA/car1 expression was analysed among developmentally regulated genes in the UV-C response.
"Expression of developmentally regulated genes yakA, car1, aca, csA, regA, ctnA, ctnB, gp24, hspD and dsn were analysed using semiquantitative RT-PCR"
Leaps and lulls in the developmental transcriptome of Dictyostelium discoideum.
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Starving cells communicate via extracellular cAMP and aggregate into multicellular organisms.
"communicate via extracellular cAMP, aggregate by tens of thousands and develop into multicellular organisms"
Curcumin inhibits development and cell adhesion in Dictyostelium discoideum: Implications for YakA signaling and GST enzyme function.
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Curcumin delays chemotaxis, adhesion and development in Dictyostelium.
"This resulted in delayed chemotaxis, adhesion, and development of the organism"
The Legionella autoinducer LAI-1 is delivered by outer membrane vesicles to promote interbacterial and interkingdom signaling.
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Legionella LAI-1, delivered via outer membrane vesicles, inhibits Dictyostelium migration; the paper does not identify cAR1 as a receptor or ligand.
"inhibit the migration of Dictyostelium discoideum amoeba"
Collective signalling drives rapid jumping between cell states.
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A global gene-expression jump coincides with the onset of collective oscillations of cAMP, sensed via cAR1.
"the jump coincides with the onset of collective oscillations of cAMP"
The Ras association domain of DydA as a specific reporter of activated RasG in Dictyostelium.
Localization of cyclic-AMP receptors with acidosomes in Dictyostelium discoideum.
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Internalized cAR1 localizes with acidosomes, proposed to be early/ recycling endosome-like compartments.
"acidosomes contain abundant receptors for cyclic AMP (CAR1) and that it may be the site for recycling of internalized receptors"
Two cAMP receptors activate common signaling pathways in Dictyostelium.
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cAR1 is coupled by a heterotrimeric G protein to intracellular effectors and normally mediates cAMP responses; cAR1/cAR3 are partially redundant.
"cAR1, coupled by a typical heterotrimeric G-protein to intracellular effectors"
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cAR1 and cAR3 interact with the same heterotrimeric G protein.
"both interact with the same heterotrimeric G-protein to mediate these and other responses"
Phospholipase C in Dictyostelium discoideum. Identification of stimulatory and inhibitory surface receptors and G-proteins.
Dual role of cAMP and involvement of both G-proteins and ras in regulation of ERK2 in Dictyostelium discoideum.
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cAMP binding to cAR1 transiently activates ERK2 in a G-protein-dependent manner.
"Binding of extracellular cAMP to cAMP receptor 1, a G-protein coupled cell surface receptor, transiently stimulates phosphorylation, activation and nuclear translocation of ERK2"
Random mutagenesis of the cAMP chemoattractant receptor, cAR1, of Dictyostelium. Mutant classes that cause discrete shifts in agonist affinity and lock the receptor in a novel activational intermediate.
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cAR1 transduces extracellular cAMP via G protein-dependent and -independent mechanisms; mutants alter cAMP binding.
"The cAMP chemoattractant receptor, cAR1, of Dictyostelium transduces extracellular cAMP signals via G protein-dependent and G protein-independent mechanisms"
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A mutant class showed a conditional cAMP-binding defect reversed by high salt.
"One class had a conditional defect in cAMP binding that was reversed by high salt"
Identification of detergent-resistant plasma membrane microdomains in dictyostelium: enrichment of signal transduction proteins.
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cAR1 is enriched in detergent-resistant plasma-membrane microdomains, confirmed as plasma-membrane in origin.
"Cell surface biotinylation and preembedding immunoelectron microscopy both confirmed the plasma membrane origin of this preparation"
Switching of chemoattractant receptors programs development and morphogenesis in Dictyostelium: receptor subtypes activate common responses at different agonist concentrations.
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cAR1 is the first of four sequentially expressed cAMP receptors and mediates chemotaxis, adenylyl cyclase activation and actin polymerization.
"assessed the capacity of each to mediate chemotaxis, activation of adenylyl cyclase and actin polymerization"
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The Dictyostelium cAMP receptor family comprises four receptors (cAR1-cAR4) expressed sequentially during development.
"The cAMP chemoattractant receptor family of Dictyostelium discoideum is composed of four receptors (cAR1-cAR4)"
Developmentally and spatially regulated activation of a Dictyostelium STAT protein by a serpentine receptor.
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Extracellular cAMP acting through cAR1 rapidly induces Dd-STAT tyrosine phosphorylation and nuclear translocation.
"The tyrosine phosphorylation and nuclear localization of Dd-STAT are induced very rapidly by extracellular cAMP through the serpentine cAMP receptor cAR1"