High-throughput phenotypic characterization of Pseudomonas aeruginosa membrane transport genes.
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PA1256, PA1257 and PA1260 form a predicted ABC amino acid transporter operon.
"PA1256, PA1257 and PA1260 are all components of a predicted ABC amino acid transporter encoded in a putative operon along with two hypothetical genes."
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Knockouts are defective for growth on hydroxy-L-proline and the genes are induced by it, but not by L-proline.
"Knockouts in these genes affected growth on hydroxy-L-proline as a carbon source, and expression of all three genes was induced by hydroxy-L-proline (Table 2 and 3)."
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The phenotype is specific to hydroxy-L-proline.
"There was no growth defect on L-proline, nor was expression affected by L-proline as an inducer, suggesting specificity for hydroxy-L-proline."
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods