Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
Targeting of hFis1 to peroxisomes is mediated by Pex19p.
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PEX11A-YFP was used as a peroxisomal membrane marker in COS-7 cells, confirming its peroxisomal localization.
"Pex11pα-YFP, which exposes its N and C termini toward the cytosol ( 53 ), localized specifically to peroxisomes ( Fig"
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PEX11A-YFP did not induce morphological alterations of peroxisomes, unlike PEX11B, consistent with its lower basal activity.
"In contrast to other peroxisomal membrane proteins (for example, Pex11pβ) ( 46 ), no morphological alterations of the peroxisomal compartment were induced by Pex11pα-YFP, which might have influenced the association of hFis1 ( Fig"
The peroxisomal membrane protein import receptor Pex3p is directly transported to peroxisomes by a novel Pex19p- and Pex16p-dependent pathway.
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HA-PEX11alpha was used as a control in Pex16p knockdown experiments and was correctly targeted to peroxisomes even when Pex3p was misdirected.
"We confirmed the complementing activity of PEX14-EGFP, HA-PEX13, HA-PEX12, HA-PEX10, HA-PEX2, and PEX3-Myc, using respective PEX-defective mutants, and the peroxisome division activity of HA-PEX11alpha with wild-type CHO cells."
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PEX11A served as negative control for Pex16p-mediated Pex3p recruitment, showing its targeting is independent of Pex16p.
"We confirmed the complementing activity of PEX14-EGFP, HA-PEX13, HA-PEX12, HA-PEX10, HA-PEX2, and PEX3-Myc, using respective PEX-defective mutants, and the peroxisome division activity of HA-PEX11alpha with wild-type CHO cells."
PEX11 family members are membrane elongation factors that coordinate peroxisome proliferation and maintenance.
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PEX11 family members (including PEX11A) are membrane elongation factors that drive formation of juxtaposed elongated peroxisomes (JEPs).
"Ectopic expression of proteins of the PEX11 family from yeast, plant or human lead to the formation of juxtaposed elongated peroxisomes (JEPs),which is evocative of an evolutionary conserved function of these proteins in membrane tubulation."
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PEX11A homo- and heterodimerizes (with PEX11G) and interacts with FIS1.
"We established the homo- and heterodimerization properties of the human PEX11 proteins and their interaction with the fission factor hFis1"
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JEPs are intermediates in peroxisome membrane proliferation; FIS1 is the limiting factor for progression to fission.
"Our results demonstrate that PEX11-induced JEPs represent intermediates in the process of peroxisome membrane proliferation and that hFis1 is the limiting factor for progression."
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Excess FIS1 but not DRP1 is sufficient to resolve JEPs into round organelles.
"We show that excess of hFis1 but not of DRP1 is sufficient to fragment JEPs into normal round-shaped organelles"
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Microtubules are required for JEP formation.
"We show that excess of hFis1 but not of DRP1 is sufficient to fragment JEPs into normal round-shaped organelles, and illustrate the requirement of microtubules for JEP formation."
Clofibrate-inducible, 28-kDa peroxisomal integral membrane protein is encoded by PEX11.
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Human PEX11 (PEX11A) encodes a 247 amino acid peroxisomal integral membrane protein with two transmembrane segments.
"PEX11 encoded a peroxisomal protein Pex11p comprising 247 amino acids, with two transmembrane segments and a dilysine motif at the C-terminus."
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PEX11A is identical to the clofibrate-inducible 28 kDa peroxisomal integral membrane protein (PMP28).
"Pex11p comigrated in SDS-PAGE with a 28-kDa peroxisomal integral membrane protein (PMP28) isolated from the liver of clofibrate-treated rats and was crossreactive to anti-PMP28 antibody, thereby indicating PEX11 to encode PMP28."
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N- and C-terminal parts are exposed to the cytosol.
"Pex11p exposes both N- and C-terminal parts to the cytosol."
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PEX11 was not responsible for any of ten tested complementation groups of peroxisome deficiency disorders.
"PEX11 was not responsible for ten complementation groups of human peroxisome deficiency disorders."
Expression of PEX11beta mediates peroxisome proliferation in the absence of extracellular stimuli.
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PEX11alpha overexpression induces peroxisome proliferation but at lower frequency than PEX11beta.
"Overexpression of PEX11alpha also induced peroxisome proliferation but at a much lower frequency than PEX11beta in our experimental system."
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PEX11alpha mRNA is induced more than 10-fold by peroxisome proliferators clofibrate and DEHP.
"PEX11alpha mRNA levels varied widely among different tissues, were highest in tissues that are sensitive to peroxisome-proliferating agents, and were induced more than 10-fold in response to the peroxisome proliferators clofibrate and di(2-ethylhexyl) phthalate."
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PEX11alpha mRNA levels are highest in tissues sensitive to peroxisome-proliferating agents.
"PEX11alpha mRNA levels varied widely among different tissues, were highest in tissues that are sensitive to peroxisome-proliferating agents"
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PEX11beta is constitutively expressed while PEX11alpha is inducible.
"Overexpression of the human PEX11beta gene alone was sufficient to induce peroxisome proliferation, demonstrating that proliferation can occur in the absence of extracellular stimuli and may be mediated by a single gene."
Peroxisome synthesis in the absence of preexisting peroxisomes.
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PEX11alpha-myc was used as a peroxisomal membrane protein marker in PEX16-deficient cell studies.
"Similar results were observed in cells transfected with expression vectors designed to express other human PMPs, including PEX3myc (Kammerer et al., 1998), PEX10myc (Warren et al., 1998), PEX11αmyc (Schrader et al., 1998), PEX11βmyc (Schrader et al., 1998), and PEX13myc (Gould et al., 1996) (data not shown)"
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Two pathways proposed for peroxisome formation - PEX11-mediated division of pre-existing peroxisomes and PEX16-mediated de novo formation.
"We propose that peroxisomes may form by either of two pathways: one that involves PEX11-mediated division of preexisting peroxisomes, and another that involves PEX16-mediated formation of peroxisomes in the absence of preexisting peroxisomes."