Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location Vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Proteome analysis of the human mitotic spindle.
Defining the membrane proteome of NK cells.
Mdp3 is a novel microtubule-binding protein that regulates microtubule assembly and stability.
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MAP7D3 (Mdp3) binds tubulin and microtubules via N-terminal coiled-coil domains
"Serial deletion experiments showed that the two coiled coil motifs of Mdp3 were critical for its interaction with tubulin and microtubules."
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Regulates cellular microtubule assembly and enhances microtubule stability
"Cold recovery and nocodazole washout assays further demonstrated an important role for Mdp3 in regulating cellular microtubule assembly. Our data also showed that Mdp3 significantly enhanced the stability of cellular microtubules."
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Expression varies during cell cycle with highest levels in G1, S, and M phases
"we found that Mdp3 expression varied during the cell cycle and in primary tissues."
C-terminal region of MAP7 domain containing protein 3 (MAP7D3) promotes microtubule polymerization by binding at the C-terminal tail of tubulin.
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C-terminal MDCT fragment binds microtubules with Kd ~3.0 uM
"MDCT bound to reconstituted microtubules with an apparent dissociation constant of 3.0 ± 0.5 µM."
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Promotes microtubule polymerization and stabilizes against dilution-induced disassembly
"MDCT stabilized microtubules against dilution induced disassembly."
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Binds tubulin C-terminal tail and competes with tau for binding sites
"Competition experiments with tau indicated that MDCT shares its binding site on microtubules with tau."
Deep research report on MAP7D3