Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Characterization of the Pseudomonas aeruginosa Lol system as a lipoprotein sorting mechanism.
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Purified P. aeruginosa LolCDE, reconstituted in proteoliposomes, releases lipoproteins to LolA in an ATP-dependent manner; LolB then incorporates them into the outer membrane.
"When incubated in the presence of ATP and a LolA homologue, the reconstituted LolCDE homologue released lipoproteins, leading to the formation of a LolA-lipoprotein complex."
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P. aeruginosa LolCDE does not release lipoproteins carrying the Lys3-Ser4 inner-membrane retention signal.
"As revealed in vivo, lipoproteins with Lys and Ser at positions 3 and 4, respectively, remained in proteoliposomes."