Gene Ontology annotation through association of InterPro records with GO terms
Use of the ND evidence code for Gene Ontology (GO) terms
A human protein-protein interaction network: a resource for annotating the proteome.
Large-scale proteomics and phosphoproteomics of urinary exosomes.
In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.
A reference map of the human binary protein interactome.
ARL15, a GTPase implicated in rheumatoid arthritis, potentially repositions its truncated N-terminus as a function of guanine nucleotide binding.
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ARL15 hydrolyses GTP with measured kinetics.
"Using spectroscopy, we demonstrated that ARL15 exhibits properties inherent of GTPases. The Km and Vmax of the enzyme were calculated to be 100 μM and 1.47 μmole/min/μL, respectively."
Structural insights into regulation of CNNM-TRPM7 divalent cation uptake by the small GTPase ARL15.
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ARL15 binds GTP and CNNM2; crystal structure of the complex.
"The crystal structure of a complex between ARL15 and CNNM2 CBS-pair domain reveals the molecular basis for binding and allowed the identification of mutations that specifically block binding."
ARL15 modulates magnesium homeostasis through N-glycosylation of CNNMs.
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ARL15 negatively regulates Mg2+ transport via CNNM N-glycosylation.
"Altogether, our results establish ARL15 as a novel negative regulator of Mg2+ transport by promoting the complex N-glycosylation of CNNMs."
Arl15 upregulates the TGFβ family signaling by promoting the assembly of the Smad-complex.
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Active ARL15 binds Smad4 MH2 and relieves its autoinhibition.
"The binding relieves the autoinhibition of Smad4, which is imposed by the intramolecular interaction between its MH1 and MH2 domains."
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ARL15 localizes to the Golgi and endolysosome.
"Arl15 localizes to the Golgi and endolysosome."
Palmitoylated small GTPase ARL15 is translocated within Golgi network during adipogenesis.
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Palmitoylated ARL15 moves between Golgi compartments in adipocytes.
"ARL15 was predominantly co-localised with a marker of the cis face of Golgi at the preadipocyte stage and then translocated to other Golgi compartments after differentiation was induced."
Golgi Localized Arl15 Regulates Cargo Transport and Cell Adhesion.
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ARL15 regulates cargo transport from the Golgi.
"Collectively, these studies implicate a functional role for Arl15 in regulating cargo transport from the Golgi to regulate cell surface processes."
Cleft palate and minor metabolic disturbances in a mouse global Arl15 gene knockout.
S-acylation and membrane localization of the small GTPase ARL15 are mediated by the Golgi S-acyltransferases ZDHHC7 and ZDHHC3.
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ARL15 S-acylation by ZDHHC7/ZDHHC3 anchors it to membranes.
"Loss of S-acylation disrupted membrane association of ARL15, as shown by confocal imaging and subcellular fractionation."
Structural insights into regulation of TRPM7 divalent cation uptake by the small GTPase ARL15.
The metabolic syndrome- associated small G protein ARL15 plays a role in adipocyte differentiation and adiponectin secretion.
UniProt entry Q9NXU5 (ARL15_HUMAN)
Affinage mechanistic annotation for ARL15 (human)