Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
The silencing protein SIR2 and its homologs are NAD-dependent protein deacetylases.
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HST2 catalyzes NAD-dependent histone deacetylation with absolute requirement for NAD cofactor
"these enzymes also catalyze histone deacetylation in a reaction that absolutely requires NAD, thereby distinguishing them from previously characterized deacetylases"
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Family activity is distinct from previously characterized histone deacetylases
"these enzymes also catalyze histone deacetylation in a reaction that absolutely requires NAD, thereby distinguishing them from previously characterized deacetylases"
A phylogenetically conserved NAD+-dependent protein deacetylase activity in the Sir2 protein family.
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NAD-dependent deacetylase activity is phylogenetically conserved across sirtuin family
"This HDA activity is also displayed by purified yeast Sir2p and homologous Archaeal, eubacterial, and human proteins, and depends completely on NAD(+) in all species tested."
A cytosolic NAD-dependent deacetylase, Hst2p, can modulate nucleolar and telomeric silencing in yeast.
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HST2 is predominantly cytoplasmic (distinct from nuclear SIR2 and HST1)
"Here we report that yeast Hst2p and a mammalian Hst2p homologue, hSirT2p, are cytoplasmic in yeast and human cells, in contrast to yHst1p and ySir2p which are exclusively nuclear"
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HST2 overexpression increases rDNA repression while derepressing subtelomeric silencing
"overexpression of yHst2p influences nuclear silencing events in a SIR2 strain, derepressing subtelomeric silencing while increasing repression in the rDNA"
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HST2 can influence nuclear silencing events through extra-nuclear localization
"Although yHst2p cannot restore silencing in a sir2 deletion, overexpression of yHst2p influences nuclear silencing events in a SIR2 strain"
HST2 mediates SIR2-independent life-span extension by calorie restriction.
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HST2 mediates lifespan extension independent of SIR2 under calorie restriction
"Sir2-independent life-span extension is mediated by Hst2, a Sir2 homolog that promotes the stability of repetitive ribosomal DNA"
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HST2 promotes stability of repetitive ribosomal DNA sequences
"Here, we show that Sir2-independent life-span extension is mediated by Hst2, a Sir2 homolog that promotes the stability of repetitive ribosomal DNA, the same mechanism by which Sir2 extends life span"
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HST2 extends lifespan through same DNA stability mechanism as SIR2
"Here, we show that Sir2-independent life-span extension is mediated by Hst2, a Sir2 homolog that promotes the stability of repetitive ribosomal DNA, the same mechanism by which Sir2 extends life span"
SirT2 is a histone deacetylase with preference for histone H4 Lys 16 during mitosis.
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HST2 (yeast ortholog) exhibits strong preference for histone H4K16Ac as deacetylation substrate
"SirT2 and its yeast counterpart Hst2 have a strong preference for histone H4K16Ac in their deacetylation activity in vitro and in vivo"
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H4K16Ac deacetylation is important for chromatin condensation during cell cycle
"The enzymatic conversion of H4K16Ac to its deacetylated form may be pivotal to the formation of condensed chromatin"
Nuclear export modulates the cytoplasmic Sir2 homologue Hst2.
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HST2 shuttles between nucleus and cytoplasm
"Hst2 moves between the nucleus and cytoplasm, but is largely cytoplasmic owing to efficient nuclear export"
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HST2 is largely cytoplasmic due to efficient CRM1-mediated nuclear export
"This nuclear exclusion is mediated by the exportin chromosomal region maintenance 1 (Crm1) and a putative leucine-rich nuclear export sequence in Hst2"
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Nuclear export regulates HST2's transcriptional repression activity
"Disruption of Hst2 export shows that nuclear exclusion inhibits the activity of Hst2 as a transcriptional repressor"
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Contains putative leucine-rich nuclear export sequence overlapping autoregulatory helix
"This nuclear exclusion is mediated by the exportin chromosomal region maintenance 1 (Crm1) and a putative leucine-rich nuclear export sequence in Hst2, which overlaps a unique autoregulatory helix"
14-3-3 Protein Bmh1 triggers short-range compaction of mitotic chromosomes by recruiting sirtuin deacetylase Hst2.
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Bmh1 recruits phosphorylated Hst2 to mitotic chromatin to drive H4K16ac removal and chromatin compaction.
"Bmh1-mediated recruitment of phosphorylated Hst2 to mitotic chromatin induces chromatin compaction. Phosphorylation of H3 tails by mitotic kinases creates a binding site for 14-3-3 protein Bmh1, which in turn elicits recruitment of Hst2, leading to removal of H4K16ac and chromatin compaction."
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Hst2 S320/S324 phosphorylation controls Bmh1 interaction in vivo.
"Our results show that phosphorylation of Hst2 S320 or S324 is necessary and sufficient to induce the interaction with Bmh1."
The cellular economy of the Saccharomyces cerevisiae zinc proteome.
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This study identified the yeast zinc proteome with global-domain and local-motif bioinformatic searches.
"The yeast zinc proteome of 582 known or potential zinc-binding proteins was identified using a bioinformatics analysis that combined global domain searches with local motif searches."
UniProt entry for HST2/NAD-dependent protein deacetylase HST2
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UniProt summarizes HST2 catalytic activity, zinc cofactor, and localization.
"NAD-dependent histone deacetylase that is involved in nuclear silencing events. Cytoplasm. Nucleus."
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UniProt records Hst2 as binding one zinc ion per subunit.
"Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit."
Falcon deep research report for HST2
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Falcon synthesis supports H4K16 deacetylation as the most precise Hst2 function.
"The most precise, experimentally supported biological role for budding-yeast Hst2 is to promote short-range mitotic chromosome compaction through H4K16 deacetylation."
PANTHER family PTHR11085 NAD-dependent sirtuin protein deacylase metadata