Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic Gene Ontology annotation based on Rhea mapping
Combined Automated Annotation using Multiple IEA Methods
Growth signalling pathways in Arabidopsis and the AGC protein kinases.
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Kinase activity by similarity (AGC kinase review).
"The Arabidopsis AGC kinases contain sequence motives required for the docking of PDK1 and phosphorylation of their activation loop in the kinase catalytic domain."
A protein kinase target of a PDK1 signalling pathway is involved in root hair growth in Arabidopsis.
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Physical interaction of D6PK with PDK1. The cached abstract describes AGC2-1 (OXI1) as the PDK1 target studied; the D6PK (AGC1-1) interaction presumably comes from additional assays in the full text, which was not checked. GO:0005515 protein binding conveys no molecular function.
"AGC2-1 specifically interacts with AtPDK1 through a conserved C-terminal hydrophobic motif"
Structural and functional insights into the regulation of Arabidopsis AGC VIIIa kinases.
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Physical interaction of D6PK with PDK1 (AGCVIIIa kinases are PDK1 substrates). GO:0005515 protein binding conveys no molecular function.
"the majority of Arabidopsis AGCVIIIa kinases are substrates for the 3-phosphoinositide-dependent kinase 1 (PDK1)"
The polarly localized D6 PROTEIN KINASE is required for efficient auxin transport in Arabidopsis thaliana.
Arabidopsis D6PK is a lipid domain-dependent mediator of root epidermal planar polarity.
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D6PK at the plasma membrane of root epidermal cells.
"D6PK undergoes phosphatidylinositol-4,5-bisphosphate- and sterol-dependent basal-to-planar polarity switching into the polar, lipid-enriched domain just before hair formation"
Roles of AGCVIII Kinases in the Hypocotyl Phototropism of Arabidopsis Seedlings.
Extensive signal integration by the phytohormone protein network.
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Physical interaction of D6PK with BREVIS RADIX (BRX) in a large-scale phytohormone Y2H interactome. GO:0005515 protein binding conveys no molecular function.
"we experimentally generated a systems-level map of the Arabidopsis phytohormone signalling network"
AGC kinases and MAB4/MEL proteins maintain PIN polarity by limiting lateral diffusion in plant cells.
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Physical interaction of D6PK with MEL1/NPY5, an NPH3-family PIN polarity adaptor. GO:0005515 protein binding conveys no molecular function.
"Indeed, HIS-D6PK was clearly pulled down with GST-MEL1"
Auxin efflux by PIN-FORMED proteins is activated by two different protein kinases, D6 PROTEIN KINASE and PINOID.
Phospholipid composition and a polybasic motif determine D6 PROTEIN KINASE polar association with the plasma membrane and tropic responses.
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D6PK bound PI(4,5)P2 in lipid-binding assays. D6PK binds polyacidic phospholipids rather broadly through a polybasic lysine-rich motif, which targets it to the plasma membrane.
"We further show that D6PK directly binds polyacidic phospholipids through a polybasic lysine-rich motif in the middle domain of the kinase."
D6PK AGCVIII kinases are required for auxin transport and phototropic hypocotyl bending in Arabidopsis.
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D6PK-family kinases are required for first and second positive hypocotyl phototropism.
"phototropic hypocotyl bending is strongly dependent on the activity of D6PKs and the PIN proteins PIN3, PIN4, and PIN7"
Figure 5: Direct D6PK interaction with phosphatidylinositol phosphates in vitro. | Nature Plants | Nature Plants
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The binding experiment uses GST control and GST-D6PK overlays on defined lipid spots.
"Protein-lipid overlay assay of lipid strips with 0.5 µg ml–1 GST (b) or GST–D6PK (c) detected by anti-GST antibody."
D6PK plasma membrane polarity requires a repeated CXX(X)P motif and PDK1-dependent phosphorylation.