ERG12 (YMR208W) notes

UniProt P07277; mevalonate kinase, EC 2.7.1.36; GHMP kinase family; cytosolic [UniProt:P07277].

Evidence journal

• Gene/enzyme: PMID:1645230; identical to RAR1; feedback: PMID:1645230.
• Activity at native levels: PMID:16477420
• Genetics: PMID:200835 ergosterol auxotrophs.
• Localisation: cytoplasm/nucleus (Huh 2003 GFP), cytosol (SWAT, PMID:26928762) [UniProt:P07277 "Cytoplasm, cytosol"].

Decisions

• The PAINT IBAs from PTN000048555 for mevalonate kinase activity, cytosol, and IPP
  biosynthesis via mevalonate are sound: ERG12 is in the single PTHR43290 mevalonate-kinase
  subfamily and retains the EC 2.7.1.36 reaction.
• The fungal PTN000825284 ergosterol-biosynthesis IBA is sound for budding yeast; the
  PAINT node is seeded by S. cerevisiae ERG12, and Karst & Lacroute show that erg12 mutants
  are ergosterol auxotrophs lacking mevalonate kinase.
• The broad Huh 2003 and InterPro2GO cytoplasm rows should be refined to the cytosol
  already supported by SWAT, PAINT, UniProtKB-SubCell, and YeastPathways rows.
• The broad isoprenoid biosynthetic process rows are true but too general beside Erg12's
  direct GO:0019287 mevalonate-to-IPP role.
• ATP binding, Mg2+ binding (ISS from rat MVK) and nucleus HDA are KEEP_AS_NON_CORE.

2026-10-31 rescue

• The original codex/erg12-yeast-20261024 worktree had been left on an obsolete
  origin/main base with only truncated generated cache CSVs modified. The cache changes
  were discarded and the review branch was fast-forwarded to current origin/main.
• just fetch-gene yeast ERG12 and just fetch-gene-pmids yeast ERG12 found no GOA,
  UniProt, or publication-cache changes.
• Newer searches found metabolic-engineering uses of ERG12, but no post-2024 paper that
  changes the native mevalonate kinase, cytosol, IPP-biosynthesis, or ergosterol-biosynthesis
  calls in the cached evidence.