Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Combined Automated Annotation using Multiple IEA Methods
Bioactivation of 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB 1954) by human NAD(P)H quinone oxidoreductase 2: a novel co-substrate-mediated antitumor prodrug therapy.
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NQO2 activity is latent unless supplied with a reduced dihydronicotinamide co-substrate such as NRH; NAD(P)H is not the physiological electron donor.
"its activity is normally latent, and a nonbiogenic co-substrate such as NRH [nicotinamide riboside (reduced)] is required for enzymatic activity"
Towards a proteome-scale map of the human protein-protein interaction network.
OpenScientist blinded function-assignment report for NQO2 (NAD(P)H dehydrogenase (quinone) activity, GO:0003955)
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The existing OpenScientist report recommends removal based on NRH preference. The biochemical re-review agrees on the preferred donor but does not adopt the inference that inefficient NAD(P)H usage means no catalytic capacity.
"recommend REMOVAL of the IBA GO:0003955"
Crystal structure of quinone reductase 2 in complex with resveratrol.
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Resveratrol is a potent (Kd ~35 nM) inhibitor of QR2 that binds in the active-site cleft, with all three of its hydroxyl groups hydrogen-bonding to QR2 residues.
"resveratrol is a potent inhibitor of quinone reductase 2 (QR2) activity in vitro with a dissociation constant of 35 nM"
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The resveratrol molecule is anchored in the QR2 active site via hydrogen bonds from all three of its hydroxyl groups.
"All three resveratrol hydroxyl groups form hydrogen bonds with amino acids from QR2"
Kinetic, thermodynamic and X-ray structural insights into the interaction of melatonin and analogues with quinone reductase 2.
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QR2 is the cytosolic MT3 melatonin binding site; melatonin is a competitive active-site inhibitor seen in multiple orientations in the QR2 dimer.
"melatonin binds in multiple orientations within the active sites of the QR2 dimer as opposed to an allosteric site"
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QR2 is a high-affinity (nanomolar) target of resveratrol, with a determined QR2:resveratrol crystal structure.
"It is one of the most potently inhibited (nanomolar) molecular targets of resveratrol, a natural polyphenol found in wine and peanuts, and the X-ray structure of QR2 in complex with resveratrol has been determined"
Large-scale proteomics and phosphoproteomics of urinary exosomes.
In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.
Cochaperone binding to LYR motifs confers specificity of iron sulfur cluster delivery.
A proteome-scale map of the human interactome network.
Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.
A reference map of the human binary protein interactome.
NQO2:FAD dimer reduces quinones to hydroquinones
Catalytic properties of NAD(P)H:quinone oxidoreductase-2 (NQO2), a dihydronicotinamide riboside dependent oxidoreductase.
The Unusual Cosubstrate Specificity of NQO2: Conservation Throughout the Amniotes and Implications for Cellular Function.