Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Combined Automated Annotation using Multiple IEA Methods
Secretory carrier membrane proteins interact and regulate trafficking of the organellar (Na+,K+)/H+ exchanger NHE7.
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SCAMP2 directly interacts with NHE7 via its TM2-TM3 cytoplasmic loop
"the C-terminal cytoplasmic tail of NHE7 bound preferentially to a highly conserved cytoplasmic loop between the second and the third transmembrane segments (TM2-TM3 loop) of SCAMP2"
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SCAMP2-NHE7 complexes localize primarily to TGN with minor fraction in recycling vesicles
"The majority of the NHE7-SCAMP complexes accumulated at the TGN, but a minor fraction also resided in recycling vesicles."
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SCAMPs participate in shuttling NHE7 between recycling vesicles and the TGN
"We propose a model wherein SCAMPs participate in the shuttling of NHE7 between recycling vesicles and the TGN."
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Deletion mutant SCAMP2/Delta184-208 causes redistribution of NHE7 to scattered recycling vesicles
"A deletion mutant of SCAMP2 lacking this region (SCAMP2/Delta184-208) bound weakly to NHE7, but caused a significant fraction of NHE7 and wild-type SCAMP2 to redistribute to a pool of scattered recycling vesicles"
Subcellular redistribution of the serotonin transporter by secretory carrier membrane protein 2.
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SCAMP2 interacts with SERT via the N-terminal domain
"GST-pulldown assays confirmed the physical interaction between SCAMP2 and the N-terminal domain of SERT."
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Co-expression causes subcellular redistribution of SERT with decreased cell surface expression
"Co-expression of SERT and SCAMP2 in mammalian cells results in the subcellular redistribution of SERT with a decrease in cell surface SERT"
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SCAMP2-SERT interaction causes reduced 5-HT uptake activity
"a decrease in cell surface SERT and a concomitant reduction in 5-HT uptake activity"
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Cys201Ala mutation in SCAMP2 E peptide abolishes SERT down-regulation but not binding
"A single amino acid mutation, cysteine-201 to alanine, within the conserved cytoplasmic E peptide of SCAMP2, abolished SCAMP2-mediated down-regulation of SERT, although this mutation had no effect on the physical interaction between SERT and SCAMP2."
Secretory Carrier Membrane Protein 2 Regulates Cell-surface Targeting of Brain-enriched Na+/H+ Exchanger NHE5.
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NHE5 directly binds to N- and C-terminal cytosolic extensions of SCAMP2
"In vitro protein-protein interaction assays revealed that NHE5 directly binds to the N- and C-terminal cytosolic extensions of SCAMP2."
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SCAMP2 (but not SCAMP5) increases NHE5 cell-surface abundance and activity
"Heterologous expression of SCAMP2 but not SCAMP5 increased cell-surface abundance as well as transporter activity of NHE5 across the plasma membrane."
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SCAMP2-mediated NHE5 surface targeting is Arf6-dependent but Rab11-independent
"SCAMP2-mediated surface targeting of NHE5 was reversed by dominant-negative Arf6 but not by dominant-negative Rab11."
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SCAMP2 and NHE5 co-localize predominantly in recycling endosomes
"secretory carrier membrane proteins (SCAMPs), a group of tetraspanning integral membrane proteins that reside in multiple secretory and endocytic organelles, bind to NHE5 and co-localize predominantly in the recycling endosomes."
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
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SCAMP2 identified in B-cell exosome proteome (high-throughput mass spectrometry)
"we first analyzed the total proteome of highly purified B cell-derived exosomes using sensitive and accurate mass spectrometry (MS), and identified 539 proteins"
A reference map of the human binary protein interactome.
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HuRI systematic Y2H screening identified SCAMP2 interactions
"Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'. With approximately 53,000 protein-protein interactions"
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
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SCAMP2-FLNA interaction identified in neurodegenerative disease interactome
"Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains"
Three mammalian SCAMPs (secretory carrier membrane proteins) are highly related products of distinct genes having similar subcellular distributions.
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Three human SCAMP forms cloned and characterized
"The primary structures of three human forms of secretory carrier membrane proteins (SCAMPs) have been deduced from full-length clones"
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SCAMPs show extensive colocalization within cells
"Examination of the codistribution of the three forms within individual cells using double label immunofluorescence indicates extensive colocalization"
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SCAMPs function in post-Golgi recycling pathways
"These findings suggest that the SCAMPs may largely function at the same sites during vesicular transport rather than in separate post-Golgi recycling pathways."
Deep research review of SCAMP2 function
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SCAMP2 is a tetraspanning membrane protein involved in post-Golgi trafficking
"SCAMP family proteins, including SCAMP2, are implicated in the regulation of membrane trafficking steps that govern exocytosis and endocytosis/recycling"