Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Combined Automated Annotation using Multiple IEA Methods
Interaction of the bacteriophage T4 gene 59 helicase loading protein and gene 41 helicase with each other and with fork, flap, and cruciform DNA.
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Helicase loader gp59 binds to and stimulates gp41 DNA helicase activity on Holliday junctions and strand-invasion-like branched DNA substrates.
"Consistent with the roles of these proteins in recombination, we find that 59 protein binds to and stimulates 41 helicase activity on Holliday junction DNA, and on a substrate that resembles a strand invasion structure."
Bacteriophage T4 helicase loader protein gp59 functions as gatekeeper in origin-dependent replication in vivo.
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Origin-replicative intermediates are completely absent in T4 infections lacking the gp41 replicative helicase.
"We also found that all origin-replicative intermediates were absent in infections deficient in the helicase gp41 or the single-strand-binding protein gp32, regardless of whether gp59 was present or absent."
Kinetic parameters of the translocation of bacteriophage T4 gene 41 protein helicase on single-stranded DNA.
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gp41 possesses a single-stranded DNA-dependent ATPase activity that drives processive translocation along ssDNA and is essential for its helicase activity.
"The bacteriophage T4 gene 41 helicase protein (gp41) carries a single-stranded DNA-dependent ATPase activity that is essential to its helicase activity."
UniProtKB record for P04530 (HELI_BPT4)
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gp41 is an essential 5'-3' hexameric ATP-dependent DNA helicase that unwinds duplex DNA ahead of the replication fork and interacts with the primase in the primosome complex.
"ATP-dependent DNA helicase essential for viral DNA replication and recombination (PubMed:10871615). The helicase moves 5' -> 3' on the lagging strand template, unwinding the DNA duplex ahead of the leading strand polymerase at the replication fork and generating ssDNA for both leading and lagging strand synthesis (PubMed:11459969, PubMed:23578280)."
Deep research summary for Enterobacteria phage T4 replicative helicase gp41
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gp41 is a hexameric ring-shaped replicative helicase that couples ATP/GTP hydrolysis to 5'-3' translocation on ssDNA and unwinding of replication forks.
"**gp41 is the T4 replicative helicase** and functions as an **ssDNA-stimulated ATPase/GTPase** whose NTP hydrolysis drives **5′→3′ translocation** on ssDNA and **fork unwinding**."