Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana.
Proteomic study of the Arabidopsis thaliana chloroplastic envelope membrane utilizing alternatives to traditional two-dimensional electrophoresis.
D-GLYCERATE 3-KINASE, the last unknown enzyme in the photorespiratory cycle in Arabidopsis, belongs to a novel kinase family.
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GLYK purified from leaves is encoded by single-copy At1g80380; recombinant enzyme is a glycerate kinase without PRK or uridine kinase activity; knockouts lack activity, accumulate glycerate and require elevated CO2; GLYK founds a novel kinase family shared with fungi and some cyanobacteria.
"These data demonstrate that At GLYK represents the only significant source of GLYK activity in Arabidopsis."
Sorting signals, N-terminal modifications and abundance of the chloroplast proteome.
Quantitative proteomics of a chloroplast SRP54 sorting mutant and its genetic interactions with CLPC1 in Arabidopsis.
AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins.
Photorespiration.
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GLYK catalyses the final ATP-dependent phosphorylation of glycerate to 3-phosphoglycerate; single-copy gene; knockout is conditionally lethal; not light induced.
"Knock-out of this gene results in a conditionally lethal photorespiratory phenotype."
A proteomic strategy for global analysis of plant protein complexes.
Global Analysis of Membrane-associated Protein Oligomerization Using Protein Correlation Profiling.
Light Controls Protein Localization through Phytochrome-Mediated Alternative Promoter Selection.
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Phytochrome-regulated alternative promoter selection yields a cytoplasmic GLYK isoform in shade that acts as a photorespiratory bypass.
"Cytoplasmic GLYK constitutes a photorespiratory bypass that alleviates fluctuating light-induced photoinhibition."