Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Disparate subcellular localization patterns of Pseudomonas aeruginosa Type IV pilus ATPases involved in twitching motility.
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PilB and PilT localize to both poles; PilB polar localization requires the inner-membrane protein PilC.
"PilB and PilT fusions localized to both poles, while PilU fusions localized only to the piliated pole."
Multiple roles of the pilus biogenesis protein pilD: involvement of pilD in excretion of enzymes from Pseudomonas aeruginosa.
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PilB function is restricted to pilus biogenesis; pilB mutants do not have the protein export defect of pilD mutants.
"The function of the other two accessory proteins, PilB and PilC, appears to be restricted to pilus biogenesis"
Products of three accessory genes, pilB, pilC, and pilD, are required for biogenesis of Pseudomonas aeruginosa pili.
Functional role of conserved residues in the characteristic secretion NTPase motifs of the Pseudomonas aeruginosa type IV pilus motor proteins PilB, PilT and PilU.
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Purified P. aeruginosa PilB has ATPase activity in vitro, and Walker A, Walker B, Asp box and His box residues are required for twitching.
"Assay of purified 6xHis-tagged PilB, PilT and PilU from P. aeruginosa showed that all three proteins have ATPase activities in vitro."
A phage protein that inhibits the bacterial ATPase required for type IV pilus assembly.
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Phage D3112 protein Tip binds PilB and inhibits its activity, abolishing piliation and twitching motility.
"Tip interacts with and blocks the activity of bacterial-encoded PilB, the TFP assembly/extension ATPase"
Competitive binding of independent extension and retraction motors explains the quantitative dynamics of type IV pili.
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PilB is the only known extension motor in P. aeruginosa; PilT is the main retraction motor.
"PilB is the only known extension motor, PilT is considered the primary retraction motor, and PilU has been shown to affect retraction"
UniProt entry P22608 (PILB_PSEAE)