Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
SpoIIQ anchors membrane proteins on both sides of the sporulation septum in Bacillus subtilis.
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SpoIIE localizes to forespore compartment after cytokinesis
"SpoIIE is released from the septum and transiently localizes to all membranes in the forespore compartment"
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SpoIIE specifically re-localizes to septal membrane on forespore side during engulfment
"Upon the initiation of engulfment, it specifically re-localizes to the septal membrane on the forespore side"
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SpoIIQ is required for anchoring SpoIIE at the engulfing septum
"the re-localization of SpoIIE to the engulfing septum requires SpoIIQ"
Protein-tyrosine phosphorylation interaction network in Bacillus subtilis reveals new substrates, kinase activators and kinase cross-talk.
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SpoIIE interacts with RacA and RecA by yeast two-hybrid
"RacA interaction with YabT and SpoIIE by yeast 2HB. Gal4 BD- (line) and AD-fusions (column) expressed in yeast haploid cells of complementary mating type were mated and assayed for expression of interaction phenotype"
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SpoIIE can dephosphorylate RacA in vitro
"In vitro phosphorylation/dephosphorylation of RacA by YabT, and SpoIIE. Phosphorylation was performed as described in the experimental procedures"
Deep research summary for spoIIE from Falcon literature search
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SpoIIE is a PP2C-family Mn2+-dependent phosphatase that dephosphorylates SpoIIAA-P
"SpoIIE is a PP2C-family, Mn2+-dependent serine/threonine protein phosphatase. Its primary physiological substrate is SpoIIAA-P"
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Mn2+ promotes SpoIIE oligomerization and is required for phosphatase activity
"Mn2+ not only supports catalysis but also promotes SpoIIE oligomerization into large assemblies"
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SpoIIE localizes to polar Z-rings and modulates asymmetric division
"SpoIIE localizes to polar Z-rings and influences Z-ring stability and septal wall architecture"
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SpoIIE shows high specificity for SpoIIAA-P over non-cognate substrates
"SpoIIE exhibits pronounced specificity for its cognate STAS-domain substrate, SpoIIAA-P, over non-cognate RsbV-P"