GIPC3 manual evidence and species boundaries

HuRI source pairs

PMID:32296183(https://pubmed.ncbi.nlm.nih.gov/32296183/), HuRI.psi SHA256: d6e8185519d18ce18cfa66f5eab66ecb1e711113f0b62810e0a145dd33a9ee12.

ORF 54149 has partial DNA identification. Three method projections (MI:0397/MI:1112/MI:1356) per pair are not independent experiments. Official ST6GAL2 and PLEKHA2 cross-references resolve identifiers without proving full clone equivalence.

MYO6 binding and orthology

Mouse constructs bind MYO6 in purified pulldowns and MYO18A in cellular assays. Simultaneous force coupling remains a model. Results report HA tagging; Methods specify MBP.

Original locators: final article, MYO6 Results, purification/pulldown Methods, Figure 3 and supplementary Table S1 (page 9). Other supplementary panels were not inspected.

The official Ensembl orthology response identifies a one-to-one relationship. The versionless Ensembl identifiers map to the following versioned official UniProt cross-references.

The retained PTHR12259 member table places human Q8TF64 and mouse Q8R5M0 in PTHR12259:SF2, distinct from the GIPC1 and GIPC2 subfamilies. Its mouse length is 297 residues, agreeing with the full-length mouse construct in Table S1. This classification corroborates the independent orthology call; it is not an experimental activity annotation.

This verifies orthology, not direct human activity. The proposed GO:0070853 ISO annotation requires prior mouse IPI curation. The retained donor snapshot has ND roots only. The GO annotation guide, WITH/FROM section, calls for an experimental donor annotation; changing ISO to ISS would not resolve that omission. The current GORULE:0000038 is proposed, report-only and soft, not a hard validation gate. The prerequisite here is a conservative curation ordering.

Mouse localization is not directly established in human hair cells. Broader myosin binding would add no independent GO coverage.

Human genetics and products

PMID:21326233(https://pmc.ncbi.nlm.nih.gov/articles/PMC3105340/) combines mouse auditory phenotypes and rescue with human recessive variants. Its trafficking discussion is mechanistic background, not a direct binding assay. The unresolved 37-kDa mouse band does not define a human isoform.

PMID:21660509(https://pmc.ncbi.nlm.nih.gov/articles/PMC3303183/) reports seven human variant families, six with LOD scores above 3. Original Methods and Table 1 concern clinical evaluation, linkage and sequencing; no construct assay was performed. Mouse and GIPC1 mechanisms in its Discussion are cited background.

PMID:11836571(https://doi.org/10.3892/ijo.20.3.577) remains abstract-only: human fetal-lung cloning and RNA expression establish identity, while related-protein signaling examples do not establish GIPC3 pathways. The official human record specifies one 312-residue product; its AAC24304.1 warning concerns a separate erroneous genomic prediction. Engineered mouse deletions and patient variants are not additional natural products.

Retained donor annotation snapshot

The official QuickGO query, captured on 2026-11-05 at 12:01:50 UTC, returned the two records below. The exact response objects are retained under donor_annotation_snapshot in the source archive. This dated snapshot is not a new mouse review or an assertion that the literature lacks experimental evidence.