Gene Ontology annotation based on Enzyme Commission mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Combined Automated Annotation using Multiple IEA Methods
The T4 phage UvsW protein contains both DNA unwinding and strand annealing activities.
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UvsW is a 3'-5' helicase active on stalled replication forks and recombination intermediates that also possesses an intrinsic single-stranded DNA annealing activity enhanced by ATP hydrolysis.
"UvsW is a 3' to 5' helicase that unwinds a wide variety of substrates, including those resembling stalled replication forks and recombination intermediates. UvsW also contains a potent single-strand DNA annealing activity that is enhanced by ATP hydrolysis but does not require it."
Bacteriophage T4 UvsW protein is a helicase involved in recombination, repair and the regulation of DNA replication origins.
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Purified UvsW displays ssDNA-dependent ATPase and branched-DNA helicase activities that are abolished by the Walker A K141R mutation.
"In support of this model, a partially purified GST/UvsW fusion protein, but not a GST/UvsW-K141R fusion, displays ssDNA-dependent ATPase activity and is able to unwind a branched DNA substrate."
UniProtKB record for P0DXF1 (UVSW_BPT4)
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UvsW functions in recombination-dependent DNA repair, stalled replication fork reorganization, and Holliday junction branch migration.
"Plays important roles in recombination-dependent DNA repair and the reorganization of stalled replication forks during viral DNA synthesis (PubMed:9233823, PubMed:2388264)."
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UvsW processes Holliday junction intermediates by catalyzing branch migration.
"Helps process Holliday junction (HJ) intermediates to mature products by catalyzing branch migration (PubMed:17823128, PubMed:23732982)."
Deep research summary for Enterobacteria phage T4 helicase uvsW
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UvsW is a superfamily 2 3'-5' branch-specific helicase and DNA-rewinding motor that remodels R-loops, replication forks, and Holliday junctions.
"**Primary function:** UvsW is an ATP-dependent, SF2, 3′→5′ **branch-specific helicase/rewinding motor** that remodels R-loops, replication forks, Holliday junctions, and recombination intermediates."