Annotation inferences using phylogenetic trees
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Two novel RING-type ubiquitin ligases, RGLG3 and RGLG4, are essential for jasmonate-mediated responses in Arabidopsis.
The Arabidopsis Iron-Sulfur Protein GRXS17 is a Target of the Ubiquitin E3 Ligases RGLG3 and RGLG4.
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GRXS17 is a substrate of AtRGLG3/RGLG4; UBC30 is a cognate E2. In vitro, auto-ubiquitination was shown for RGLG3 and GRXS17 ubiquitination with UBC30; GRXS17 degradation in planta depends on RGLG3 and RGLG4.
"GRXS17 is ubiquitinated and degraded in an RGLG3- and RGLG4-dependent manner in planta"
Hijacking of the jasmonate pathway by the mycotoxin fumonisin B1 (FB1) to initiate programmed cell death in Arabidopsis is modulated by RGLG3 and RGLG4.
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GFP-RGLG4 and FLAG-RGLG4 are found in both cytoplasm and nucleus by confocal imaging and cell fractionation.
"Confocal images showed that GFP-RGLG3 and GFP-RGLG4 were present in both the cytoplasm and nucleus"
The E3 ubiquitin ligase OsRGLG5 targeted by the Magnaporthe oryzae effector AvrPi9 confers basal resistance against rice blast.
OpenScientist hypothesis investigation - E3 ubiquitin ligase activity as core function
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GO:0061630 strongly supported. Recommend upgrading from GO:0004842 (parent E2+E3 term) to GO:0061630 (E3-specific term). Multiple IDA-level demonstrations in closest ortholog AtRGLG4 and family-wide conservation.
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Q6YYC5 has a myristoylation-compatible N-terminus (MG motif) suggesting possible plasma membrane association, qualifying CC annotations but not core MF.
Deep research report for Q6YYC5 Os08g0135400 RGLG family E3 ligase
Q6YYC5 re-review sequence comparison with Arabidopsis RGLG1-5