"To facilitate genetic analysis, I surveyed Drosophila and human sequence databases for genes predicting proteins related to GAPs for Ras superfamily members. Remarkably, close to 0.5% of genes in both species (173 human and 64 Drosophila genes) predict proteins related to GAPs for Arf, Rab, Ran, Rap, Ras, Rho, and Sar family GTPases."

"A two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions... The network recapitulated known pathways, extended pathways, and uncovered previously unknown pathway components."

"GIT1-like proteins are GTPase-activating proteins (GAPs) for Arfs and interact with a variety of signaling molecules to function as integrators of pathways controlling cytoskeletal organization and cell motility."

"we describe the characterization of a Drosophila homologue of GIT1, dGIT, and show that it is required for proper muscle morphogenesis and myotube guidance in the fly embryo."

"The dGIT protein is concentrated at the termini of growing myotubes and localizes to muscle attachment sites in late stage embryos."

"dgit mutant embryos show muscle patterning defects and aberrant targeting in subsets of their muscles."

"dgit mutant muscles fail to localize the p21-activated kinase, dPak, to their termini. dPak and dGIT form a complex in the presence of dPIX and dpak mutant embryos show similar muscle morphogenesis and targeting phenotypes to that of dgit."

"Here, we identify the scaffold G protein coupled receptor kinase 2 interacting (GIT) protein as a component of the AZ-associated cytomatrix and as a regulator of SV endocytosis."

"GIT1 and its D. melanogaster ortholog, dGIT, are shown to directly associate with the endocytic adaptor stonin 2/stoned B."

"In Drosophila dgit mutants, stoned B and synaptotagmin levels are reduced and stoned B is partially mislocalized."

"Moreover, dgit mutants show morphological and functional defects in SV recycling. These data establish a presynaptic role for GIT in SV recycling and suggest a connection between the AZ cytomatrix and the endocytic machinery."

"we used proteomics to identify proteins that bind to the Hippo (Hpo) kinase. Prominent among these were PAK-interacting exchange factor (known as Pix or RtGEF) and G-protein-coupled receptor kinase-interacting protein (Git)."

"Git is the single Drosophila melanogaster homolog of the mammalian GIT1 and GIT2 proteins, which were originally identified in the search for molecules that interact with G-protein-coupled receptor kinases."

"Pix and Git form an oligomeric scaffold to facilitate sterile 20-like kinase activation and have also been linked to GTPase regulation."

"We show that Pix and Git regulate Hippo-pathway-dependent tissue growth in D. melanogaster and that they do this in parallel to the known upstream regulator Fat cadherin."

"Pix and Git influence activity of the Hpo kinase by acting as a scaffold complex, rather than enzymes, and promote Hpo dimerization and autophosphorylation of Hpo's activation loop."

"dGit, a Drosophila ortholog of Git1, shows remarkable conservation with mammalian Git1 both structurally and functionally. dGit forms signaling complex with dPix and dPak, and the dGit-dPix-dPak signaling pathway plays an essential role for the development in Drosophila."

"Consistent with Git1-/- mice, dGitex21C Drosophila mutants also exhibited severely decreased central brain size"

"Moreover, the development of alpha- or/and beta-lobe of the mushroom body, which is known to be important for learning and memory in Drosophila [30], was significantly affected by the deletion of dGit"

"subcellular localization and activation of the dPix-Rac-dPak signaling complex is modulated by dGit, and dysregultion of this signaling complex causes severe defects, leading to brain size reduction and abnormal mushroom body development."

"severe developmental deficits shown in Git1-/- mice as well as adult dGit1ex21C Drosophila mutants indicate the conserved and critical role of GIT1 in brain development."

"Vap33 physically and genetically interacts with the actin cytoskeletal regulators RtGEF (Pix) and Git, which also bind to the Hippo protein (Hpo) and are involved in the activation of the Hippo pathway."

"Altogether, our data suggest that Lgl acts via Vap33 to activate the Hippo pathway by a dual mechanism: (1) through interaction with RtGEF, Git and Arf79F, and (2) through interaction and inhibition of the V-ATPase, thereby controlling epithelial tissue growth."

"Git knockdown rescues the reduced Hippo pathway target gene expression in Vha68-2 mutant clones"

"Vap33 and Lgl form a protein interaction network with RtGEF, Git, Arf79F and Hpo"

"We describe a next-generation Drosophila protein interaction map-"DPIM2"-established from affinity purification-mass spectrometry of 5,805 baits, covering the largest fraction of the Drosophila proteome"