Gene Ontology annotation through association of InterPro records with GO terms

Annotation inferences using phylogenetic trees

Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:18394898

Distinct innate immune responses to infection and wounding in the C. elegans epidermis.

NIPI-3 was first identified as a kinase required for induction of nlp-29 after D. coniospora infection

"Through a forward genetic screen, we isolated mutants that failed to induce nlp-29 expression after D. coniospora infection. We identify a kinase, NIPI-3, related to human Tribbles homolog 1"
NIPI-3 acts upstream of the MAPKK SEK-1

"We identify a kinase, NIPI-3, related to human Tribbles homolog 1, that is likely to act upstream of the MAPKK SEK-1."
NIPI-3 is required specifically for infection-induced but not wound-induced nlp-29 expression

"We find NIPI-3 is required only for nlp-29 induction after infection and not after wounding."

PMID:22470487

The pseudokinase NIPI-4 is a novel regulator of antimicrobial peptide gene expression.

Describes NIPI-3 in context of epidermal immunity pathway

"infection specifically involves the Tribbles-like kinase NIPI-3"
Infection specifically involves the Tribbles-like kinase NIPI-3

"infection specifically involves the Tribbles-like kinase NIPI-3"

PMID:27927200

Tribbles ortholog NIPI-3 and bZIP transcription factor CEBP-1 regulate a Caenorhabditis elegans intestinal immune surveillance pathway.

NIPI-3 is essential for host survival following exposure to P. aeruginosa or ToxA

"the Tribbles pseudokinase ortholog nipi-3, is essential for host survival following exposure to P. aeruginosa or ToxA"
NIPI-3 is a negative regulator of CEBP-1

"NIPI-3 is a negative regulator of CEBP-1, which in turn negatively regulates protective immune mechanisms"

PMID:27927209

Coordinated inhibition of C/EBP by Tribbles in multiple tissues is essential for Caenorhabditis elegans development.

nipi-3 null mutants arrest at L2-L3 and die within 5-10 days

"Mutants arrested development at the second to third larval stages (L2-L3) (see below) and eventually died between 5-10 days after hatching."
NIPI-3 physically interacts with CEBP-1 via yeast two-hybrid

"One prominent candidate interacting partner was CEBP-1, a member of the C/EBP family of transcription factors"
Loss of cebp-1 completely suppresses nipi-3 null lethality

"Remarkably, cebp-1(0) completely suppressed the growth and fertility defects of nipi-3(0) mutants"
NIPI-3 represses cebp-1 transcription in multiple tissues

"Strikingly, the expression of the cebp-1 transcriptional reporter was highly and significantly increased in both nipi-3(fr4) and nipi-3(0) mutants, compared with wild-type animals"
NIPI-3 localizes predominantly to nuclei in epidermis, intestine, and neurons

"GFP::NIPI-3 expression was observed predominantly in the nuclei (Fig. 1i), with an overall intensity peaking at the L2-L3 stages."
NIPI-3 negatively modulates PMK-1 phosphorylation via CEBP-1

"levels of active PMK-1 were significantly increased in nipi-3(0), but remained similar to wild type in cebp-1(0) and cebp-1(0) nipi-3(0) animals"
Loss of PMK-1 pathway components also suppresses nipi-3 null lethality

"Among the other suppressors of nipi-3(0), we found missense alterations in TIR-1/the sterile alpha (SAM) and Toll-interleukin receptor (TIR) motif-containing protein SARM), NSY-1/MAPKKK, SEK-1/MAPKK and MAK-2/MAPK-activated protein kinase (MAPKAPK)"

PMID:34407394

Tribbles pseudokinase NIPI-3 regulates intestinal immunity in Caenorhabditis elegans by controlling SKN-1/Nrf activity.

NIPI-3 is a positive regulator of SKN-1 activity

"another positive regulator of SKN-1 is identified: NIPI-3, a Tribbles pseudokinase"
NIPI-3 negatively regulates CEBP-1 which positively regulates vhp-1

"NIPI-3 has been reported to protect against intestinal infection by negatively regulating the CCAT enhancer binding protein (C/EBP) bZIP transcription factor CEBP-1"
VHP-1 is a phosphatase that dephosphorylates PMK-1

"CEBP-1 positively regulates the vhp-1 transcript, which encodes a phosphatase that dephosphorylates the p38 MAPK called PMK-1"

file:worm/nipi-3/nipi-3-deep-research-falcon.md

Deep research report on nipi-3