RfC4 (P53034) curation notes
- RfC4 (Rfc40, CG14999) is the fly ortholog of human RFC2, an AAA+ small subunit of replication factor C.
- RFC function (general): PMID:8999859; PMID:8999859.
- Fly mutants: PMID:11438670; localization PMID:11438670; checkpoint PMID:11438670. Cohesion defects are indirect: PMID:11438670.
- Elg1 complex membership: PMID:27198229.
- PMID:24204884 (Elongin/Corto, wing veins), cited by FlyBase NAS/IPI rows for all Elg1-complex subunits, does not mention RFC/Elg1/PCNA (full text searched); flagged MISCITED.
- Protein binding IPI rows (14605208, 20353594, 38944040) are all with other RFC small subunits; removed as uninformative (complex membership already captured).
- Module-wide convention (RfC4, RfC38, RfC3, CG8142): contributes_to GO:0003689 ACCEPT; GO:0005663 and GO:0031391 ACCEPT; GO:0006261/GO:0006271/GO:0006272 ACCEPT; subunit-level DNA/ATP binding and ATPase, DNA repair, cohesion, checkpoint KEEP_AS_NON_CORE; generic parents (GO:0006260, GO:0008094, GO:0032991) MODIFY to the specific term the gene already carries (round-2 rule).
- Deep research: falcon run launched; see deep-research file if present.
- Deep research (falcon) arrived after first commit; confirms nuclear localization (>95% interphase cells), replication/endoreduplication defects and selective checkpoint failure in Rfc4 mutants; alternative-loader roles are conserved inference only.
- Review-bot round: added NEW contributes_to GO:0061860 DNA clamp unloader activity (IDA, PMID:27198229) so the core-function claim has an annotation row; identical across the four small subunits.