Gene Ontology annotation through association of InterPro records with GO terms
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
UniProtKB entry for PSEPK thrS
Transfer RNA-mediated editing in threonyl-tRNA synthetase. The class II solution to the double discrimination problem.
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The primary abstract identifies a distinct N-terminal ThrRS editing site that hydrolyzes mischarged Ser-tRNA(Thr). This supports the conserved editing interpretation; full-text details were not recovered in the normal PMID cache.
"hydrolysis of the incorrectly formed ser-tRNA(Thr) is performed at a specific site in the N-terminal domain of the enzyme."
Substrate specificity and catalysis by the editing active site of Alanyl-tRNA synthetase from Escherichia coli.
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Purified E. coli AlaRS editing assays distinguish mischarged Ser-tRNA(Ala) hydrolysis from cognate-product hydrolysis; the paper also discusses differences from ThrRS editing. This informs conserved architecture, not KT2440 kinetics.
"The wild-type AlaRS editing domain deacylated Ser-tRNA(Ala)"
The central role of transfer RNAs in mistranslation.