GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

GO_REF:0000123

YeastPathways to GO-CAM conversion

file:yeast/ERG19/ERG19-deep-research-falcon.md

Deep research report (falcon / Edison Scientific Literature) for yeast ERG19/MVD1

Independent synthesis confirming the ATP- and Mg2+-dependent decarboxylation of mevalonate-5-diphosphate to IPP, GHMP-kinase mechanism, cytosolic homodimeric localization, and essentiality; corroborates the core MF/BP/CC calls and the vacuole over-annotation flag. Adds regulatory and metabolic-engineering context as non-core.

PMID:14562095

Global analysis of protein localization in budding yeast.

Genome-wide GFP-fusion localization study; MVD1/ERG19 localizes to the cytoplasm.

PMID:15169949

Identification of active site residues in mevalonate diphosphate decarboxylase: implications for a family of phosphotransferases.

Asp302 is a crucial catalytic residue (D302A reduces kcat ~10^5-fold); Lys18 influences ATP binding. Places MDD in the GHMP kinase/phosphotransferase family.

PMID:1779710

Sterol pathway in yeast. Identification and properties of mutant strains defective in mevalonate diphosphate decarboxylase and farnesyl diphosphate synthetase.

Isolation of yeast erg19 mutants blocked in mevalonate diphosphate decarboxylase, auxotrophic for ergosterol.

PMID:28904410

Recent Advances in Ergosterol Biosynthesis and Regulation Mechanisms in Saccharomyces cerevisiae.

Review of ergosterol biosynthesis organized into mevalonate, farnesyl-PP and ergosterol modules.

PMID:8626466

Molecular cloning and expression of the cDNAs encoding human and yeast mevalonate pyrophosphate decarboxylase.

Cloned and expressed the yeast (and human) enzyme; recombinant enzyme is a homodimer of 43-kDa subunits with diphosphomevalonate decarboxylase activity.

PMID:9244250

The Saccharomyces cerevisiae mevalonate diphosphate decarboxylase is essential for viability, and a single Leu-to-Pro mutation in a conserved sequence leads to thermosensitivity.

ERG19 is an essential gene; disruption is lethal. A single Leu79->Pro change causes a thermosensitive MDD; overexpression lowers steady-state sterol.

PMID:11698677

Structural genomics of enzymes involved in sterol/isoprenoid biosynthesis.

Crystal structure of yeast diphosphomevalonate decarboxylase (PDB 1FI4, 2.27 A); the enzyme is a homodimer with a GHMP-kinase-like two-domain fold.