Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Combined Automated Annotation using Multiple IEA Methods
UniProtKB record for P04521 (EXO1_BPT4)
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gp47 is subunit 1 of the two-subunit T4 exonuclease complex (with gp46) involved in viral genome replication, DNA recombination, and host DNA degradation.
"FUNCTION: Exonuclease that plays a role in viral genome replication, DNA recombination, and host DNA degradation."
Deep research summary for Enterobacteria phage T4 gene 47 (gp47)
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gp47 is the Mre11-family metallophosphoesterase nuclease subunit that forms the T4 MR complex with gp46 to resect double-strand breaks for homologous recombination and recombination-dependent replication.
"The target protein specified by UniProt accession **P04521** corresponds to **gene product 47 (gp47)** from **Enterobacteria phage T4** and is consistently identified in the literature as the **T4 Mre11 homolog** (nuclease subunit) that partners with **gp46 (Rad50 homolog)** to form the **Mre11–Rad50 (MR) complex**."
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Interaction with gp32 and UvsY enables Mg2+-dependent endonucleolytic and exonucleolytic processing by the gp46/gp47 complex, coupling resection to recombinase loading.
"Specifically, while Mg2+ alone supports little to no detectable nuclease activity, the presence of **UvsY + gp32** enables a **Mg2+-dependent reaction** with a distinct product distribution (consistent with a switch toward endonucleolytic processing products under those conditions)."