Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Division of labour: tRNA methylation by the NSun2 tRNA methyltransferases Trm4a and Trm4b in fission yeast.
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Trm4b does not carry out physiological tRNA wobble C34 methylation.
"Trm4b methylates both C34 and C49 in vitro, even though it does not methylate C34 in vivo."
UniProtKB O13935: curated sequence, features and source attribution
Pmt1, a Dnmt2 homolog in Schizosaccharomyces pombe, mediates tRNA methylation in response to nutrient signaling.
Falcon research on Trm4b/O13935 tRNA methylation
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The biochemical and genetic assignments must distinguish in-vitro from physiological C34 activity.
"It does **not** overturn the in-vivo assignment of C34 to Trm4a."
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Nuclear evidence does not refute a cytoplasmic pool.
"This conclusion does not exclude transient cytoplasmic residence or modification of recycled tRNAs"