Falcon deep research on xoxG (C5B121) cytochrome c electron acceptor for lanthanide-dependent methanol dehydrogenase
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"XoxG is a **periplasmic, heme c–binding cytochrome** purified from the periplasmic fraction with a **cleaved signal peptide**, and it functions as the **physiological electron acceptor** for lanthanide-dependent XoxF methanol dehydrogenase activity"
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"is genomically adjacent to **xoxF1** (META1_1740) and **xoxJ** (META1_1742), forming an **xoxF–xoxG–xoxJ** module"
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"The mature N-terminus was identified as **Gln27 after cleavage of the periplasmic signal peptide**"
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"The heme is attached via the canonical **CXXCH** motif (Cys95/Cys98)"
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"The heme iron is axially ligated by **His99 and Met143**"
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"Em = +172 ± 1 mV at pH 7.0"
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"This is substantially lower than a cited value for the Ca-dependent partner cytochrome MxaG (**~+256 mV**)"
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"loss of **xoxG** yields a growth defect equivalent to loss of both **xoxF1 and xoxF2**, consistent with XoxG being essential for XoxF-dependent methanol oxidation"
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"Km for XoxG increased ~3-fold from La to Nd"
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"the functional model is that XoxG transfers electrons onward into the respiratory chain"