Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
F-box/WD-repeat proteins pop1p and Sud1p/Pop2p form complexes that bind and direct the proteolysis of cdc18p.
-
Pop1 and Pop2 are non-redundant, interdependent F-box/WD-repeat proteins that form heterooligomers; Cdc18 binding to Pop2 requires Pop1, and the Pop2 N-terminus fused to Pop1 rescues the pop1 pop2 double mutant.
"binding of Cdc18p to Pop2p was dependent on Pop1p"
Combinatorial diversity of fission yeast SCF ubiquitin ligases by homo- and heterooligomeric assemblies of the F-box proteins Pop1p and Pop2p.
-
Endogenous Pop1, Pop2, Pcu1, Psh1 and Pip1 form a ~500 kDa SCF(Pop1-Pop2) complex that polyubiquitylates CDK-phosphorylated Rum1 in vitro in a pop2-dependent reaction; Pop2 is nuclear and cytoplasmic, its F-box is dispensable for Rum1 degradation, and Pop1-independent SCF(Pop2) complexes with ligase activity exist.
"Rum1p ubiquitylation was not obtained with Pip1p complexes prepared from cell lysate of pop2 deletion strains, proving the F-box protein dependency of this reaction"
Requirement of the SCFPop1/Pop2 Ubiquitin Ligase for Degradation of the Fission Yeast S Phase Cyclin Cig2.
-
Pop1 and Pop2 are responsible for SCF-dependent instability of the S-phase cyclin Cig2 during G2 and M phase; Pop1 binds phosphorylated Cig2 in vivo.
"Two F-box/WD proteins Pop1 and Pop2, homologues of budding yeast Cdc4 and human Fbw7, are responsible for Cig2 instability"
Molecular interactions of fission yeast Skp1 and its role in the DNA damage checkpoint.
-
Systematic co-immunoprecipitation of twelve epitope-tagged F-box proteins with wild-type and ts Skp1; Pop2 is one of the surveyed F-box proteins.
"12 F-box proteins and Pcu1 were epitope-tagged, and co-immunoprecipitation performed"
Repression of ergosterol level during oxidative stress by fission yeast F-box protein Pof14 independently of SCF.
-
Characterisation of the F-box protein Pof14; Pop2 appears only as a reference F-box protein for Skp1 interaction and the paper's biology does not concern Pop2.
"provide substrate specificity to the SCF (Skp1, Cullin, F-box protein) E3 ubiquitin ligases by interacting with the core component Skp1 through their F-box motif"
sud1(+) targets cyclin-dependent kinase-phosphorylated Cdc18 and Rum1 proteins for degradation and stops unwanted diploidization in fission yeast.
-
sud1/pop2, a CDC4 homologue, is required to prevent spontaneous re-replication and diploidization; sud1-deleted cells cannot degrade Cdc18 or Rum1, and Sud1 binds Cdc18 only after CDK phosphorylation.
"Sud1-Cdc18 binding requires prior phosphorylation of the Cdc18 polypeptide at CDK consensus sites"
Two F-box/WD-repeat proteins Pop1 and Pop2 form hetero- and homo-complexes together with cullin-1 in the fission yeast SCF (Skp1-Cullin-1-F-box) ubiquitin ligase.
-
Cloning of pop2: Pop2 overlaps with Pop1 in Rum1 and Cdc18 degradation but the two are not functional homologues; they form hetero- and homodimers, giving three SCF species with cullin-1 as scaffold.
"By forming three distinct complexes, SCFPop1/Pop1, SCFPop1/Pop2 and SCFPop2/Pop2, SCF has evolved a sophisticated mechanism to control the level of Rum1 and Cdc18"
Falcon deep research report for SCHPO pop2
-
Synthesis identifying Pop2/Sud1 as a Cdc4-like F-box/WD-repeat substrate receptor of SCF whose best-supported targets are phosphorylated Cdc18 and Rum1.
"substrate-recruiting component of SCF ubiquitin ligases**, not an enzyme with an independently demonstrated catalytic reaction"