Annotation inferences using phylogenetic trees

Electronic Gene Ontology annotations created by ARBA machine learning models

Combined Automated Annotation using Multiple IEA Methods

SDF-9, a protein tyrosine phosphatase-like molecule, regulates the L3/dauer developmental decision through hormonal signaling in C. elegans.

sdf-9 mutants form dauer-like larvae resembling daf-9/daf-12 Daf-c mutants, enhanced by cholesterol deprivation; SDF-9 promotes DAF-9 activity.

"suggested that SDF-9 increases the activity of DAF-9 or helps the execution of the DAF-9 function"
SDF-9 is expressed in the XXXL/R cells, which also express DAF-9; ablation of these cells causes dauer-like arrest.

"Killing of the sdf-9-expressing cells in the wild-type first-stage larva induced formation of the dauer-like larva."

PMID:16839187

Two membrane-associated tyrosine phosphatase homologs potentiate C. elegans AKT-1/PKB signaling.

SDF-9 lacks the catalytic cysteine and is predicted to be catalytically inactive.

"Whereas SDF-9 does not retain the canonical catalytic cysteine residue found in all known PTPs and is therefore predicted to be catalytically inactive"
SDF-9::GFP localizes to the XXX plasma membrane independently of DAF-2 signalling.

"indicating that its localization does not require normal levels of DAF-2/InsR signaling"
Tagged SDF-9 did not co-precipitate tyrosine phosphoproteins and did not bind phosphoinositides (data not shown), so a pTyr-binding adaptor function is unproven.

"epitope-tagged SDF-9 and EAK-6 did not coprecipitate tyrosine phosphoproteins after exposure of transfected cultured 293T cells to IGF-1"
SDF-9 may act with DAF-9 in XXX to promote dafachronic acid production.

"In XXX, SDF-9 may function with DAF-9/CYP27A1 to promote the synthesis and/or secretion of dafachronic acids"

PMID:17660545

Caenorhabditis elegans SDF-9 enhances insulin/insulin-like signaling through interaction with DAF-2.

SDF-9 is proposed, from genetic interaction data, to stabilize activated DAF-2 or act as an adaptor in insulin-like signalling.

"We propose that SDF-9 stabilizes the active phosphorylated state of DAF-2 or acts as an adaptor protein to enhance insulin-like signaling."

file:worm/sdf-9/sdf-9-bioinformatics/RESULTS.md

SDF-9 catalytic-residue check against PTP1B

Catalytic Cys aligns to Ser223; P-loop His and Ser/Thr and pTyr-loop Tyr are also substituted; P-loop Arg retained.

"The catalytic cysteine position is **Ser223** in the current SDF-9 sequence."

file:worm/sdf-9/sdf-9-deep-research-wormbase.md

WormBase gene description for sdf-9 (worm)