GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

InterPro mappings supplied broad iron and molybdenum-ion annotations that require subunit- and cofactor-aware correction.

GO_REF:0000003

Gene Ontology annotation based on Enzyme Commission mapping

EC mapping assigned complete xanthine dehydrogenase activity without distinguishing subunit contribution.

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

Combined automated methods supplied broad oxidoreductase activity.

file:PSEPK/xdhB/xdhB-uniprot.txt

UniProtKB entry for Pseudomonas putida KT2440 XdhB (Q88F20)

UniProt identifies XdhB as the xanthine-dehydrogenase molybdopterin-binding subunit.

"DR InterPro; IPR014309; Xanthine_DH_Mopterin-bd_su."

file:PSEPK/xdhA/xdhA-uniprot.txt

UniProtKB entry for Pseudomonas putida KT2440 XdhA (Q88F21)

The complementary XdhA sequence, rather than XdhB, carries the specific [2Fe-2S]-binding signature.

"DR InterPro; IPR006058; 2Fe2S_fd_BS."

PMID:11341925

Xanthine dehydrogenase from Pseudomonas putida 86: specificity, oxidation-reduction potentials of its redox-active centers, and first EPR characterization.

The homologous enzyme oxidized both hypoxanthine and xanthine using NAD+ as preferred acceptor.

"oxidation of hypoxanthine, xanthine, purine, and some aromatic aldehydes, using"
The enzyme contained distinct large and small subunits.

"XDH from P. putida 86 consists of 91.0 kDa and 46.2 kDa"

PMID:26355499

Identification of a chemoreceptor that specifically mediates chemotaxis toward metabolizable purine derivatives.

KT2440 can use hypoxanthine and xanthine as sole nitrogen sources.

"permitting their use as sole nitrogen sources"

file:PSEPK/xdhB/xdhB-deep-research-openscientist.md

OpenScientist deep-research report for Pseudomonas putida KT2440 xdhB

The report correctly assigns Q88F20 to the molybdopterin-bearing catalytic subunit rather than the FAD/Fe-S subunit.

"with no FAD- or iron-sulfur-binding regions"
It explicitly identifies the target-specific evidence gap.

"No direct enzymology on Q88F20 itself."