Falcon deep research report for DENR
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The DENR-MCTS1 complex acts on post-termination 40S ribosomal subunits to facilitate removal of deacylated tRNA from the P-site, which is essential for 40S dissociation from mRNA and recycling back into the translational pool.
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DENR-MCTS1 promotes translation reinitiation, particularly after short uORFs in 5'UTRs, and this reinitiation is selective for only a subset of uORF-containing transcripts; DENR is functionally distinct from its single-chain homolog eIF2D.
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Under oxidative (arsenite) stress, DENR relocalizes together with MCTS1, eIF2D, ABCE1, eRF1 and eRF3 to cytoplasmic stress granules, suggesting spatial regulation of post-termination/reinitiation factors during stress.
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
A human protein-protein interaction network: a resource for annotating the proteome.
A novel mitochondrial ubiquitin ligase plays a critical role in mitochondrial dynamics.
MARCH-V is a novel mitofusin 2- and Drp1-binding protein able to change mitochondrial morphology.
MCT-1 protein interacts with the cap complex and modulates messenger RNA translational profiles.
A comprehensive resource of interacting protein regions for refining human transcription factor networks.
Activities of Ligatin and MCT-1/DENR in eukaryotic translation initiation and ribosomal recycling.
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MCT-1 and DENR (homologous to N- and C-terminal regions of Ligatin) together promote eIF2-independent recruitment of initiator tRNA to 40S/mRNA complexes when the initiation codon is placed directly in the P-site.
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MCT-1/DENR can promote release of deacylated tRNA and mRNA from recycled 40S subunits after ABCE1-mediated dissociation of post-termination ribosomes.
Next-generation sequencing to generate interactome datasets.
DENR-MCTS1 heterodimerization and tRNA recruitment are required for translation reinitiation.
A reference map of the human binary protein interactome.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Targeting Tau Mitigates Mitochondrial Fragmentation and Oxidative Stress in Amyotrophic Lateral Sclerosis.
Human MCTS1-dependent translation of JAK2 is essential for IFN-γ immunity to mycobacteria.
Multimodal cell maps as a foundation for structural and functional genomics.