wzx (PA3153, G3XD19) notes

Literature

No deep-research provider was available. Full texts cached for PMID:20824106, PMID:22554073 and
PMID:24023388; abstracts only for PMID:9922263 and PMID:8606190.

Integral IM protein PMID:20824106; topology resolved into cytoplasmic, TM and
periplasmic regions PMID:20824106.
Proton-coupled antiport PMID:24023388.
Mutants lose B-band LPS PMID:9922263.

PseudoCAP IBA-seed assessment

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	verdict	rationale	other seeds	IBD stands without this seed?
PTN001250140	GO:0005886 plasma membrane	5	101 (33 taxa)	GO:0005886, IDA, PMID:24023388 (PseudoCAP)	SOUND	Full text: membrane-fraction recovery of the overexpressed fusion; IM topology shown in PMID:20824106.	E. coli P0AAA7, P37746, P77377, Q46841	Yes.
PTN001250140	GO:0005886 plasma membrane	5	101 (33 taxa)	GO:0098567 periplasmic side of plasma membrane, IDA, PMID:20824106 (PseudoCAP)	WRONG	Full text: Wzx is a 12-TM integral protein with loops on both sides, not a periplasmic-face protein. PSEUDOCAP WRONG_TERM defect, CONFIRMED.	as above	Yes, on the sound GO:0005886 seed and four E. coli seeds; no GO_CENTRAL defect.

Consistent with the pmpM review (tier 2), where a periplasmic-side row on a polytopic transporter was
also judged WRONG.

Other decisions

GO:0009276 Gram-negative cell wall (PseudoCAP IDA, PMID:9922263): REMOVE, PSEUDOCAP WRONG_TERM,
PROBABLE (abstract only; independent topology data place Wzx in the IM).
GO:0016020 membrane: MODIFY to GO:0005886.
GO:0015774 polysaccharide transport (ISS) and GO:0055085 (IEA): MODIFY to GO:0034203 glycolipid
translocation (Wzx flips single lipid-linked repeat units).
GO:0043165 outer membrane assembly: KEEP_AS_NON_CORE.
GO:0015297 antiporter activity: ACCEPT; no proton-coupled flippase term exists (floppase terms are
ATP-driven). Raised as a suggested question.
Consistent with Q9HTB8 (wzm): plasma membrane accepted, generic membrane modified to plasma membrane,
O antigen biosynthesis accepted.