GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

Maps InterPro anti-sigma-factor family records to sigma factor antagonist activity, negative regulation of transcription, and sporulation-related GO terms.

GO_REF:0000033

Annotation inferences using phylogenetic trees

Phylogenetic-tree-based propagation supports SpoIIAB as an anti-sigma factor for sigma F, consistent with orthologs across Bacillus and other Firmicutes.

GO_REF:0000043

Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping

UniProt keywords ATP-binding, Kinase, Nucleotide-binding, Serine/threonine-protein kinase, Sporulation, and Transferase map to the corresponding GO terms.

"KW ATP-binding; Direct protein sequencing; Kinase; Nucleotide-binding;"

GO_REF:0000104

Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features

Manual annotations from characterized anti-sigma-factor kinases (e.g. SpoIIAB, RsbW) are propagated by shared sequence features (ATP-binding kinase domain plus anti-sigma-factor fold).

GO_REF:0000116

Automatic Gene Ontology annotation based on Rhea mapping

UniProt/Rhea catalytic-activity mapping (EC 2.7.11.1; L-seryl/L-threonyl-protein + ATP → phospho-form + ADP) supports protein serine/threonine kinase activity.

"Reaction=L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP +"

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

Consensus of multiple IEA pipelines supports serine/threonine kinase activity and ATP binding for SpoIIAB, consistent with the manually curated function.

PMID:25278935

Cross-phosphorylation of bacterial serine/threonine and tyrosine protein kinases on key regulatory residues.

SpoIIAB is one of three two-component-like serine/threonine kinases in B. subtilis (with RsbT and RsbW) surveyed for cross-phosphorylation with Hanks-type and tyrosine kinases.

"the three two-component-like serine/threonine kinases: RsbT (Kang et al., 1998), RsbW (Yang et al., 1996), and SpoIIAB (Min et al., 1993)"
SpoIIAB is among the recipient kinases most readily cross-phosphorylated by Hanks-type kinases in this in vitro survey, suggesting integration of SpoIIAB activity into a broader kinase network.

"the BY-kinase PtkA and the two-component-like kinases RsbW and SpoIIAB show the highest propensity to be phosphorylated by other kinases"