nrde-2 (G5EG51) review notes

Deep research: falcon timed out (exit 137) and the perplexity-lite fallback is
unavailable. No deep-research file was generated. The review is based on cached
publications.

Key facts with provenance

• NRDE-2 is recruited by NRDE-3/siRNA to nascent transcripts PMID:20543824.
• It inhibits RNAP II elongation PMID:20543824.
• It is required for NRDE-1 loading and for H3K9me PMID:21901112.
• It is required for the dsRNA-induced H3K9me3 footprint PMID:22231482.
• In the germline, HRDE-1 recruits NRDE-2 PMID:22810588.
• It moves to the nucleolus and inhibits RNAP I when risiRNAs are present PMID:34365510.
• H3K27me3 is also directed through the Nrde pathway (PMID:26365259; abstract only, not
  quoted in the review).
• Human NRDE2 is an MTR4/exosome inhibitor in speckles PMID:30842217. This has not been
  tested in worm.

Decisions

• protein binding (NRDE-3 IPI): REMOVE (uninformative).
• Post-transcriptional silencing IMP: MODIFY to GO:0031047 (the mechanism is
  co-transcriptional).
• mRNA stabilization ISS: MARK_AS_OVER_ANNOTATED. Nuclear speck and negative regulation
  of RNA catabolic process: kept as non-core.
• No core MF assigned. The direct molecular activity of NRDE-2 is unknown.
• I considered a NEW negative regulation of transcription elongation by RNA polymerase II
  (GO:0034244) and rejected it: the comparator check failed, because no other NRDE
  factor carries this term.