hrde-1 (Q09249) review notes

Deep research: just deep-research-falcon worm hrde-1 --fallback perplexity-lite failed
(falcon killed at the 600 s timeout, exit 137; the perplexity fallback is unavailable in
this environment). No deep-research file was generated. The review is based on cached
publications and the UniProt record.

Key facts with provenance

• HRDE-1/WAGO-9 is a germline nuclear WAGO Argonaute required for RNAi inheritance
  PMID:22810588.
• It binds 22G endo-siRNAs PMID:22810588.
• It recruits NRDE-2 to pre-mRNA PMID:22810588.
• Silencing is co-transcriptional PMID:22810588.
• It is nuclear, and also visits nuage PMID:37083324.
• WAGOs are not slicers PMID:22738726.
  Our own check (hrde-1-bioinformatics/RESULTS.md) found no DEDH tetrad residue
  conserved against human AGO2.
• It is needed for piRNA-initiated multigenerational silencing PMID:22738725.

Decisions

• RNA endonuclease IBA: REMOVE (slicer loss in the WAGO clade).
• miRNA binding IBA: MODIFY to siRNA binding. RISC IBA: MODIFY to RNAi effector complex.
• Post-transcriptional silencing (IBA and IMP): MODIFY to GO:0031048 (matches the module
  and the NRDE-2 annotation).
• Germ cell development IMP: non-core (transgenerational Mrt phenotype).