Quorum quenching by an N-acyl-homoserine lactone acylase from Pseudomonas aeruginosa PAO1.
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Purified PvdQ deacylates long-chain AHLs.
"In a bioassay, the purified acylase was shown to degrade AHLs with side chains ranging in length from 11 to 14 carbons at physiologically relevant low concentrations."
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GC and GC/MS show lauric acid released from C12-HSL by the purified enzyme.
"one can conclude that the PA2385 protein is an AHL acylase"
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No activity on the beta-lactam compounds tested.
"No activity was detected."
Identification and characterization of novel pyoverdine synthesis genes in Pseudomonas aeruginosa.
Synthesis of the siderophore pyoverdine in Pseudomonas aeruginosa involves a periplasmic maturation.
Structural characterization and high-throughput screening of inhibitors of PvdQ, an NTN hydrolase involved in pyoverdine synthesis.
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PvdQ is acylated by the acyl group derived from the acyl-pyoverdine precursor.
"Structural studies are also presented showing PvdQ covalently acylated with the acyl moiety substrate derived from the acyl-pyoverdine precursor"
High cellular organization of pyoverdine biosynthesis in Pseudomonas aeruginosa: clustering of PvdA at the old cell pole.
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Fluorescently labelled PvdQ is uniformly distributed in the periplasm.
"Epifluorescence and TIRF imaging on cells expressing labelled PvdQ showed that this enzyme was uniformly distributed in the periplasm"
The quorum-quenching N-acyl homoserine lactone acylase PvdQ is an Ntn-hydrolase with an unusual substrate-binding pocket.
Role of PvdQ in Pseudomonas aeruginosa virulence under iron-limiting conditions.
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Under iron limitation the pvdQ deletion impairs swarming and biofilm formation, effects linked to pyoverdine.
"PvdQ plays a major role in swarming motility, in biofilm development and in infection that is more likely to be linked to the pyoverdine pathway"
Influence of Pseudomonas aeruginosa pvdQ gene on altering antibiotic susceptibility under swarming conditions.
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PvdQ overproduction increases antibiotic resistance under swarming conditions.
"pvdQ plays an important role in elevating antibiotic resistance via swarm-cell differentiation and possibly other mechanisms as well"
Swarming of Pseudomonas aeruginosa is a complex adaptation leading to increased production of virulence factors and antibiotic resistance.
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A PA14 pvdQ mutant is defective in swarming.
"two virulence genes, lasB and pvdQ , were required for swarming motility"
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt