GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000033

Annotation inferences using phylogenetic trees

CGH-1 is part of a well-conserved family of DEAD-box RNA helicases (DDX6/Dhh1p subfamily) with conserved functions in P-body formation, mRNA regulation, and translational repression across eukaryotes.

GO_REF:0000043

Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping

GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping

GO_REF:0000116

Automatic Gene Ontology annotation based on Rhea mapping

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:11546739

cgh-1, a conserved predicted RNA helicase required for gametogenesis and protection from physiological germline apoptosis in C. elegans.

CGH-1 is the C. elegans ortholog of yeast Dhh1p/Ste13, human DDX6/RCK/p54, and Drosophila Me31B

"cgh-1, a conserved predicted RNA helicase required for gametogenesis and protection from physiological germline apoptosis in C. elegans."
CGH-1 is expressed specifically in the germline and early embryo

"cgh-1, a conserved predicted RNA helicase required for gametogenesis and protection from physiological germline apoptosis in C. elegans."
CGH-1 localizes to P granules

"cgh-1, a conserved predicted RNA helicase required for gametogenesis and protection from physiological germline apoptosis in C. elegans."
cgh-1 is required for both oocyte and sperm function

"cgh-1, a conserved predicted RNA helicase required for gametogenesis and protection from physiological germline apoptosis in C. elegans."
Loss of cgh-1 triggers excessive germline apoptosis - the first identified stimulus for this mechanism

"cgh-1, a conserved predicted RNA helicase required for gametogenesis and protection from physiological germline apoptosis in C. elegans."
CGH-1 protects developing oocytes from physiological germline apoptosis

"cgh-1, a conserved predicted RNA helicase required for gametogenesis and protection from physiological germline apoptosis in C. elegans."

PMID:12445390

Integrating interactome, phenome, and transcriptome mapping data for the C. elegans germline.

Large-scale interaction study identifying CGH-1 binding partners

"Integrating interactome, phenome, and transcriptome mapping data for the C. elegans germline."

PMID:14704431

A map of the interactome network of the metazoan C. elegans.

Systematic protein interaction mapping including CGH-1

"A map of the interactome network of the metazoan C. elegans."

PMID:16207815

Caenorhabditis elegans decapping proteins localization and functional analysis of Dcp1, Dcp2, and DcpS during embryogenesis.

CGH-1 localizes to P-bodies with decapping proteins

"Caenorhabditis elegans decapping proteins: localization and functional analysis of Dcp1, Dcp2, and DcpS during embryogenesis."

PMID:16247027

A complex containing the Sm protein CAR-1 and the RNA helicase CGH-1 is required for embryonic cytokinesis in Caenorhabditis elegans.

CGH-1 co-purifies with CAR-1 in an RNA-dependent ribonucleoprotein complex

"A complex containing the Sm protein CAR-1 and the RNA helicase CGH-1 is required for embryonic cytokinesis in Caenorhabditis elegans."
CGH-1 controls the localization of CAR-1

"A complex containing the Sm protein CAR-1 and the RNA helicase CGH-1 is required for embryonic cytokinesis in Caenorhabditis elegans."
CGH-1 and CAR-1 together regulate anaphase spindle structure during embryonic cytokinesis

"A complex containing the Sm protein CAR-1 and the RNA helicase CGH-1 is required for embryonic cytokinesis in Caenorhabditis elegans."
Partial depletion of CGH-1 phenocopies CAR-1 inhibition

"A complex containing the Sm protein CAR-1 and the RNA helicase CGH-1 is required for embryonic cytokinesis in Caenorhabditis elegans."
The CGH-1/CAR-1 complex is RNase-sensitive

"A complex containing the Sm protein CAR-1 and the RNA helicase CGH-1 is required for embryonic cytokinesis in Caenorhabditis elegans."

PMID:18692039

Processing bodies and germ granules are distinct RNA granules that interact in C. elegans embryos.

P-bodies and germ granules (P granules) are distinct but interacting RNA granules

"Processing bodies and germ granules are distinct RNA granules that interact in C. elegans embryos."
P-bodies contain complexes that inhibit translation

"Processing bodies and germ granules are distinct RNA granules that interact in C. elegans embryos."
CGH-1 is found in both P-bodies and P granules

"Processing bodies and germ granules are distinct RNA granules that interact in C. elegans embryos."
P-bodies mature differently in somatic vs germline blastomeres

"Processing bodies and germ granules are distinct RNA granules that interact in C. elegans embryos."

PMID:19123269

Empirically controlled mapping of the Caenorhabditis elegans protein-protein interactome network.

High-confidence protein interaction data for CGH-1

"Empirically controlled mapping of the Caenorhabditis elegans protein-protein interactome network."

PMID:19269369

nhl-2 Modulates microRNA activity in Caenorhabditis elegans.

CGH-1 interacts with NHL-2, a microRNA pathway component

"nhl-2 Modulates microRNA activity in Caenorhabditis elegans."

PMID:24367695

PAB-1, a Caenorhabditis elegans poly(A)-binding protein, regulates mRNA metabolism in germline by interacting with CGH-1 and CAR-1.

PAB-1 colocalizes with CGH-1 and CAR-1 at P-body granules

"PAB-1, a Caenorhabditis elegans poly(A)-binding protein, regulates mRNA metabolism in germline by interacting with CGH-1 and CAR-1."
PAB-1 is a component of P-bodies that interacts with CGH-1 and CAR-1

"PAB-1, a Caenorhabditis elegans poly(A)-binding protein, regulates mRNA metabolism in germline by interacting with CGH-1 and CAR-1."
CGH-1 and PAB-1 mutually affect each others localization

"PAB-1, a Caenorhabditis elegans poly(A)-binding protein, regulates mRNA metabolism in germline by interacting with CGH-1 and CAR-1."
The mRNA level of msp-152 is increased in cgh-1 mutant

"PAB-1, a Caenorhabditis elegans poly(A)-binding protein, regulates mRNA metabolism in germline by interacting with CGH-1 and CAR-1."
PAB-1 positively regulates mRNA levels in coordination with CGH-1 and CAR-1

"PAB-1, a Caenorhabditis elegans poly(A)-binding protein, regulates mRNA metabolism in germline by interacting with CGH-1 and CAR-1."

PMID:24844228

The DEAD Box RNA helicase VBH-1 is a new player in the stress response in C. elegans.

VBH-1 colocalizes with CGH-1 in large foci during heat shock

"The DEAD Box RNA helicase VBH-1 is a new player in the stress response in C. elegans."
CGH-1 localizes to stress granule-like structures during stress

"The DEAD Box RNA helicase VBH-1 is a new player in the stress response in C. elegans."
VBH-1 and CGH-1 associate with some of the same RNPs during heat shock

"The DEAD Box RNA helicase VBH-1 is a new player in the stress response in C. elegans."

PMID:25061667

Diverse functions of mRNA metabolism factors in stress defense and aging of Caenorhabditis elegans.

P-body formation requires CGH-1 (cgh-1 RNAi prevents DCAP-1 granule accumulation)

"Diverse functions of mRNA metabolism factors in stress defense and aging of Caenorhabditis elegans."
P-body components including CGH-1 are important for normal lifespan

"Diverse functions of mRNA metabolism factors in stress defense and aging of Caenorhabditis elegans."
cgh-1 RNAi affects stress granule dynamics

"Diverse functions of mRNA metabolism factors in stress defense and aging of Caenorhabditis elegans."

PMID:25261697

Translational control of the oogenic program by components of OMA ribonucleoprotein particles in Caenorhabditis elegans.

CGH-1 is an OMA-1-associated protein in oocyte RNPs

"Translational control of the oogenic program by components of OMA ribonucleoprotein particles in Caenorhabditis elegans."
CGH-1 interacts with OMA-1 in an RNA-dependent manner

"Translational control of the oogenic program by components of OMA ribonucleoprotein particles in Caenorhabditis elegans."
OMA RNPs function in translational repression of target mRNAs

"Translational control of the oogenic program by components of OMA ribonucleoprotein particles in Caenorhabditis elegans."

file:worm/cgh-1/cgh-1-deep-research-falcon.md

Falcon deep research report on cgh-1 (C. elegans)

CGH-1 is the C. elegans member of the DDX6/Dhh1 branch of DEAD-box helicases, acting as an ATP-dependent RNA-remodeling enzyme rather than a metabolic enzyme; its primary biochemical role is ATP-driven remodeling of RNA-containing complexes.

"CGH-1 is an **ATP-dependent RNA helicase/ATPase** (EC 3.6.4.13 in UniProt), but like many DEAD-box proteins, its primary biochemical role is understood as **ATP-driven remodeling of RNA-containing complexes** rather than sequence-specific catalysis of a small-molecule reaction."
CGH-1 ATPase activity is robustly stimulated by the NTL-1a MIF4G domain in the presence of poly(U) RNA and ATP, identifying RNA as the relevant substrate class and indicating cofactor-dependent regulation of its ATPase cycle.

"Direct worm biochemical evidence indicates CGH-1 has ATPase activity that can be **robustly stimulated by the MIF4G domain of NTL-1a** in the presence of **poly(U) RNA and ATP**"
CGH-1 binds core P-body / translational-control factors EDC-3, PATR-1, and CAR-1 with measured affinities, defining a conserved interaction hub on its RecA2 domain for decapping and repression partners.

"The EDC-3 FDF peptide binds the CGH-1 RecA2 region with **KD ~0.34 μM** (ITC), and pulldown/co-localization assays support interaction in vitro/in vivo (zhang2021insightintothe pages 5-7)."
In somatic tissues CGH-1 acts in PATR-1-dependent P-bodies linked to mRNA decapping/decay, whereas during oogenesis it forms PATR-1-independent storage bodies that protect a defined subset of maternal mRNAs.

"In somatic tissues, CGH-1 functions within **PATR-1–dependent P-bodies** implicated in **mRNA decapping** and decapping-mediated decay pathways (boag2008protectionofspecific pages 1-2). During oogenesis, CGH-1 instead promotes **protection/storage** of a defined subset of maternal mRNAs (boag2008protectionofspecific pages 1-2, boag2008protectionofspecific pages 7-8)."
CGH-1-associated mRNAs in oogenesis are strongly enriched for gonad-expressed and maternal transcripts (92% gonad-enriched, 85% maternal), indicating selective rather than nonspecific RNA association.

"Maternal mRNA association in oogenesis: **92%** of CGH-1–associated mRNAs are expressed mainly in the gonad and **85%** are classified as maternal (boag2008protectionofspecific pages 7-8)."
Somatic CGH-1 P-body localization depends on PATR-1: loss of patr-1 causes about a 12-fold reduction in somatic CGH-1 foci by the ~100-cell stage.

"Condensate dependence: loss of **patr-1** causes **~12-fold fewer** somatic CGH-1 foci by the ~100-cell stage, supporting PATR-1 dependence of somatic P-body CGH-1 localization (boag2008protectionofspecific pages 4-5)."
In the adult germline, CGH-1 marks perinuclear P-body condensates positioned on the cytoplasmic side of P granules, placing it at the interface between mRNA regulation and germ-granule small-RNA inheritance machinery.

"Recent imaging and spatial mapping demonstrate that CGH-1 marks **perinuclear P-body condensates positioned on the cytoplasmic side of P granules**."
CGH-1 helps route individual mRNAs between translation, storage, and decay in a developmental-context-dependent manner.

"CGH-1 helps **route mRNAs between translation, storage, and decay** depending on developmental context."