Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
The vegetative vacuole proteome of Arabidopsis thaliana reveals predicted and unexpected proteins.
MODIFIED VACUOLE PHENOTYPE1 is an Arabidopsis myrosinase-associated protein involved in endomembrane protein trafficking.
-
Recombinant MVP1 has no esterase or lipase activity in solution and plate assays.
"MVP1 did not hydrolyze p- nitrophenyl acetate"
-
MVP1 binds the myrosinase TGG2 but not TGG1 in GST pull-downs.
"we did not detect a similar interaction between MVP1:GST and TGG1, even in the tgg2-2 mutant, suggesting that MVP1 interacted with TGG2 in a highly specific manner."
A missense mutation in the vacuolar protein GOLD36 causes organizational defects in the ER and aberrant protein trafficking in the plant secretory pathway.
Arabidopsis plasmodesmal proteome.
ERMO3/MVP1/GOLD36 is involved in a cell type-specific mechanism for maintaining ER morphology in Arabidopsis thaliana.
-
ER aggregation in ermo3 is restricted to ER-body-forming cells and is suppressed by loss of NAI1.
"Depletion of NAI1 from ermo3-1 resulted in a total loss of ER-aggregates from whole-plant tissues (Figure 8)."
-
An ER/Golgi-cycling HDEL-tagged version is sufficient to maintain ER morphology.
"indicating that ERMO3-Venus-HDEL was functional enough to maintain ER morphology"
-
MVP1 co-immunoprecipitates PYK10 complex components.
"Coimmunoprecipitation analysis combined with mass spectrometry revealed that ERMO3/MVP1/GOLD36 interacts with the PYK10 complex"
Trafficking of the myrosinase-associated protein GLL23 requires NUC/MVP1/GOLD36/ERMO3 and the p24 protein CYB.
Arabidopsis GOLD36/MVP1/ERMO3 Is Required for Powdery Mildew Penetration Resistance and Proper Targeting of the PEN3 Transporter.
-
alp4, a GOLD36 allele, mistargets PEN3 within an abnormal ER and reduces penetration resistance to powdery mildew.
"Genetic mapping identified alp4 as an allele of GOLGI DEFECTS 36 (GOLD36)"
UniProt entry Q7XA74 (GDL21_ARATH)