Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Identification of a cytochrome b-type NAD(P)H oxidoreductase ubiquitously expressed in human cells.
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Human b5+b5R (NCB5OR) is a flavohemoprotein with cytochrome b5 and b5 reductase domains that binds heme, FAD and NAD(P)H, is reduced by NAD(P)H, reduces cytochrome c in vitro, and localizes to the cytosolic perinuclear space; proposed as a candidate oxygen sensor.
NCB5OR is a novel soluble NAD(P)H reductase localized in the endoplasmic reticulum.
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Recombinant NCB5OR is soluble with stoichiometric heme and FAD, colocalizes with calreticulin (ER marker), actively reduces cytochrome c, and produces superoxide only with very slow turnover, indicating it preferentially reduces substrates rather than acting as an NAD(P)H oxidase.
Erythrocytes take up carbon dioxide and release oxygen
CYB5Rs reduce MetHb to HbA
UniProt entry Q7L1T6 (NB5R4_HUMAN), Cytochrome b5 reductase 4