Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Membrane topology and roles of Pseudomonas aeruginosa Alg8 and Alg44 in alginate polymerization.
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Alg8 active-site and topology mutations support its glycosyltransferase role; Alg44 has cytoplasmic PilZ and periplasmic MFP regions and acts as a co-polymerase.
"These studies suggest that Alg44 may act as a co-polymerase in concert with Alg8, the major GT"
In vitro alginate polymerization and the functional role of Alg8 in alginate production by Pseudomonas aeruginosa.
Alginate Polymerization and Modification Are Linked in Pseudomonas aeruginosa.
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Native P. aeruginosa alg8 complementation produces highly structured biofilms compared with the nonmucoid deletion control, linking synthesis of the matrix polymer to biofilm architecture.
"The PDO300Δalg8(pBBR1MCS-5:alg8) and PDO300Δalg44(pBBR1MCS-5:alg44) strains established heterogeneous highly structured biofilms (Fig. 9)."