ureC2 encodes a predicted second urease alpha subunit in Nitrososphaera viennensis EN76. Its urease activity is inferred from EC/domain evidence and EN76 reports of two ureABC copies; direct literature for NVIE_015020 itself was not found in the Falcon report.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0009039 urease activity | IEA GO_REF:0000120 | ACCEPT | Summary: ACCEPT. UreC is the catalytic alpha subunit of urease, EC 3.5.1.5. Reason: The sequence is annotated as urease EC 3.5.1.5 and carries conserved urease alpha-subunit family signatures, including TIGR01792 and PANTHER PTHR43440. Falcon research did not find a primary paper explicitly characterizing NVIE_015020, so this ACCEPT is paralog/family inference rather than direct locus-specific evidence. Supporting Evidence: file:9ARCH/ureC2/ureC2-uniprot.txt RecName: Full=Urease; EC=3.5.1.5. file:9ARCH/ureC2/ureC2-uniprot.txt NCBIfam; TIGR01792; urease_alph; 1. file:9ARCH/ureC2/ureC2-deep-research-falcon.md The report found no retrieved primary paper explicitly naming NVIE_015020/A0A060HQC5, but comparative genomics reports two ureABC copies in EN76, consistent with a ureC paralog. |
| GO:0016151 nickel cation binding | IEA GO_REF:0000120 | ACCEPT | Summary: ACCEPT. Nickel binding is expected for urease catalytic subunits. Reason: Urease is a nickel metalloenzyme, and UniProt explicitly states that this subunit binds two nickel ions. Family research notes rare iron ureases, so this nickel annotation is accepted from the gene-specific UniProt metal-binding evidence rather than from family membership alone. Supporting Evidence: file:9ARCH/ureC2/ureC2-uniprot.txt Binds 2 nickel ions per subunit. file:interpro/panther/PTHR43440/PTHR43440-deep-research-falcon.md PTHR43440 family research supports nickel-dependent urease annotations for canonical UreC homologs with explicit Ni-binding evidence, while warning that iron urease paralogs make blanket nickel propagation unsafe. |
| GO:0016787 hydrolase activity | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: KEEP_AS_NON_CORE. Correct but less informative than urease activity. Reason: This parent hydrolase term is correct for urease chemistry but less informative than the specific GO:0009039 urease activity annotation. Supporting Evidence: file:9ARCH/ureC2/ureC2-uniprot.txt RecName: Full=Urease; EC=3.5.1.5. |
| GO:0016810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: KEEP_AS_NON_CORE. Correct parent activity for urease, but the specific urease activity is the core molecular function. Reason: The carbon-nitrogen hydrolase parent is compatible with the reaction, but the gene product is better represented by urease activity. Supporting Evidence: file:9ARCH/ureC2/ureC2-uniprot.txt Reaction=urea + 2 H2O + H(+) = hydrogencarbonate + 2 NH4(+). |
| GO:0019627 urea metabolic process | IEA GO_REF:0000104 | ACCEPT | Summary: ACCEPT. Urease directly metabolizes urea. Reason: Urea is the direct substrate of the urease reaction, so urea metabolic process is a plausible direct BP annotation for ureC2. The evidence is EC/domain/paralog inference; the report did not recover ureC2-specific expression or biochemical assays. Supporting Evidence: file:9ARCH/ureC2/ureC2-uniprot.txt Reaction=urea + 2 H2O + H(+) = hydrogencarbonate + 2 NH4(+). file:9ARCH/ureC2/ureC2-deep-research-falcon.md Urease reviews and EN76 comparative genomics support a urease-alpha paralog interpretation, but the directly regulated EN76 urease locus in recent transcriptomic work is NVIE_014740, not NVIE_015020. |
| GO:0043419 urea catabolic process | IEA GO_REF:0000041 | ACCEPT | Summary: ACCEPT. UniPathway correctly adds urea catabolic process context for a urease catalytic subunit. Reason: UniPathway is specific and mechanistically correct here: the enzyme catalyzes the single urease-route step of urea degradation. For ureC2, the curation should record that pathway placement is inferred from the conserved urease-alpha assignment and duplicated ureABC context, not from direct NVIE_015020 experiments. Supporting Evidence: file:9ARCH/ureC2/ureC2-uniprot.txt PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. file:interpro/panther/PTHR43440/PTHR43440-deep-research-falcon.md Family research identifies canonical UreC/large-subunit ureases as the catalytic subunits for urea hydrolysis, supporting urea catabolism for conserved UreC proteins. file:9ARCH/ureC2/ureC2-deep-research-falcon.md Comparative genomics reports N. viennensis EN76 has two ureABC copies, supporting a ureC2 paralog, while direct substrate-responsive expression evidence was recovered for ureC/NVIE_014740 rather than NVIE_015020. |
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