Gene Description

blaOXA-400 encodes an OXA/class D beta-lactamase in Acinetobacter baumannii. UniProt/CARD identify this entry as a beta-lactamase antibiotic-inactivation determinant; the expected core molecular function is beta-lactamase activity, not merely penicillin binding. UniProt names the protein 'Beta-lactamase OXA-400' (accession A0A097A0C1).

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0008658 penicillin binding	IEA GO_REF:0000002	MODIFY	Summary: The existing MF term is directionally correct but less informative than the family-specific AMR activity beta-lactamase activity. Reason: Replace the broad or over-specific electronic term 'penicillin binding' with beta-lactamase activity based on UniProt/CARD determinant identity and the curated ARO->GO mapping. Proposed replacements: beta-lactamase activity Supporting Evidence: file:genes/ACIBA/blaOXA-400/blaOXA-400-uniprot.txt DR CARD; ARO:3001586; OXA-400; ARO:0001004; antibiotic inactivation.
GO:0008800 beta-lactamase activity	RCA file:projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml	NEW	Summary: NEW candidate annotation from the curated ARO->GO mapping: beta-lactamase activity. Reason: The UniProt record cross-references a CARD/ARO AMR determinant, and the curated ARO->GO mapping projects the family-specific molecular function beta-lactamase activity. This focused draft accepts the candidate as a curator lead for this AMR determinant. Supporting Evidence: file:projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv A0A097A0C1 ARO:3001586 ARO:3000001 narrower RO:0002327 GO:0008800 beta-lactamase activity file:genes/ACIBA/blaOXA-400/blaOXA-400-uniprot.txt DR CARD; ARO:3001586; OXA-400; ARO:0001004; antibiotic inactivation.
GO:0046677 response to antibiotic	RCA file:projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml	NEW	Summary: NEW process annotation: blaOXA-400 is an AMR determinant involved in antibiotic response/resistance. Reason: CARD/ARO identity places this gene in an antimicrobial-resistance determinant family; response to antibiotic is the appropriate high-level GO biological process for the resistance role. Supporting Evidence: file:genes/ACIBA/blaOXA-400/blaOXA-400-uniprot.txt DR CARD; ARO:3001586; OXA-400; ARO:0001004; antibiotic inactivation.

Core Functions

blaOXA-400 hydrolyzes beta-lactam antibiotics as an OXA/class D beta-lactamase, contributing to antibiotic resistance.

Molecular Function:

beta-lactamase activity

Directly Involved In:

response to antibiotic

Supporting Evidence:

file:genes/ACIBA/blaOXA-400/blaOXA-400-uniprot.txt
DR CARD; ARO:3001586; OXA-400; ARO:0001004; antibiotic inactivation.

References

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Suggested Experiments

Experiment: Biochemically assay purified blaOXA-400 against representative antibiotic substrates for the inferred AMR family and measure loss of drug activity or target modification.

Type: in vitro enzyme assay

📚 Additional Documentation

Notes

(blaOXA-400-notes.md)

Notes: blaOXA-400

Focused AMR batch review. UniProt accession: A0A097A0C1. Source organism: Acinetobacter baumannii.

UniProt/CARD provenance: DR CARD; ARO:3001586; OXA-400; ARO:0001004; antibiotic inactivation.
ARO-to-GO candidate: GO:0008800 (beta-lactamase activity) from projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv and projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml.
This is a DRAFT focused review intended to cover the AMR annotation gap; it has not had a gene-specific deep-research pass.

blaOXA-400