Gene Description

ermA encodes an Erm-family 23S rRNA adenine-N6 methyltransferase in Aeromicrobium erythreum (strain ATCC 51598 / DSM 8599 / JCM 8359 / NBRC 15406 / NRRL B-3381). UniProt/CARD identify this entry as a macrolide-lincosamide-streptogramin resistance determinant; methylation of 23S rRNA alters the macrolide binding site on the large ribosomal subunit. UniProt names the protein 'rRNA adenine N-6-methyltransferase' (accession P09891).

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0000154 rRNA modification	IEA GO_REF:0000002	ACCEPT	Summary: The rRNA modification/methylation process is consistent with an Erm-family 23S rRNA methyltransferase. Reason: Erm enzymes confer resistance by methylating 23S rRNA; this process annotation is biologically appropriate. Supporting Evidence: file:genes/AERER/ermA/ermA-uniprot.txt CC -!- FUNCTION: Involved in erythromycin resistance.
GO:0000179 rRNA (adenine-N6,N6-)-dimethyltransferase activity	IEA GO_REF:0000120	ACCEPT	Summary: The existing dimethyltransferase term is retained as the more specific molecular function for this Erm-family enzyme. Reason: GO:0000179 is a child of the broader rRNA adenine-N6 methyltransferase activity and captures the N6,N6-dimethylation activity expected for Erm-mediated macrolide-lincosamide-streptogramin resistance. Supporting Evidence: file:genes/AERER/ermA/ermA-uniprot.txt CC -!- FUNCTION: Involved in erythromycin resistance.
GO:0005829 cytosol	IEA GO_REF:0000118	ACCEPT	Summary: The cellular location is compatible with the encoded AMR enzyme and is retained. Reason: The location is consistent with the protein type and existing UniProt/GOA evidence.
GO:0031167 rRNA methylation	IEA GO_REF:0000118	ACCEPT	Summary: The rRNA modification/methylation process is consistent with an Erm-family 23S rRNA methyltransferase. Reason: Erm enzymes confer resistance by methylating 23S rRNA; this process annotation is biologically appropriate. Supporting Evidence: file:genes/AERER/ermA/ermA-uniprot.txt CC -!- FUNCTION: Involved in erythromycin resistance.
GO:0046677 response to antibiotic	RCA file:projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml	NEW	Summary: NEW process annotation: ermA is an AMR determinant involved in antibiotic response/resistance. Reason: CARD/ARO identity places this gene in an antimicrobial-resistance determinant family; response to antibiotic is the appropriate high-level GO biological process for the resistance role. Supporting Evidence: file:genes/AERER/ermA/ermA-uniprot.txt DR CARD; ARO:3000594; ErmR; ARO:0001001; antibiotic target alteration.

Core Functions

ermA methylates adenine N6 in 23S rRNA, modifying the antibiotic target site in the large ribosomal subunit and contributing to macrolide/lincosamide/streptogramin resistance.

Molecular Function:

rRNA (adenine-N6,N6-)-dimethyltransferase activity

Directly Involved In:

response to antibiotic

Cellular Locations:

cytosol

Supporting Evidence:

file:genes/AERER/ermA/ermA-uniprot.txt
CC -!- FUNCTION: Involved in erythromycin resistance.

References

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Suggested Experiments

Experiment: Biochemically assay purified ermA against representative antibiotic substrates for the inferred AMR family and measure loss of drug activity or target modification.

Type: in vitro enzyme assay

📚 Additional Documentation

Notes

(ermA-notes.md)

Notes: ermA

Focused AMR batch review. UniProt accession: P09891. Source organism: Aeromicrobium erythreum (strain ATCC 51598 / DSM 8599 / JCM 8359 / NBRC 15406 / NRRL B-3381).

UniProt/CARD provenance: DR CARD; ARO:3000594; ErmR; ARO:0001001; antibiotic target alteration.
ARO-to-GO candidate: GO:0008988 (rRNA (adenine-N6-)-methyltransferase activity) from projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv and projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml.
This is a DRAFT focused review intended to cover the AMR annotation gap; it has not had a gene-specific deep-research pass.

ermA