id: A0A2G9RZF1 gene_symbol: A0A2G9RZF1 taxon: id: NCBITaxon:8400 label: Aquarana catesbeiana status: COMPLETE description: >- A0A2G9RZF1 is a single-CUB fragment from a fragmented Aquarana catesbeiana draft gene model. The ProtNLM2 single-fertilization prediction is refuted for the sequence supplied to the model, because that sequence lacks the S1 protease domain required for an ovochymase-like egg-envelope protease; extracellular-region localization remains plausible but unresolved for the exact short accession. source_documents: - genes/AQUCT/A0A2G9RZF1/A0A2G9RZF1-uniprot.txt - genes/AQUCT/A0A2G9RZF1/A0A2G9RZF1-goa.tsv - publications/PMID_8510165.md - genes/AQUCT/A0A2G9RZF1/A0A2G9RZF1-ai-review.yaml - genes/AQUCT/A0A2G9RZF1/A0A2G9RZF1-hypotheses/core-function-1-go-0005201/openscientist.md - genes/AQUCT/A0A2G9RZF1/A0A2G9RZF1-hypotheses/prediction-ovochymase-fertilization/openscientist.md predictions: - source_method: ProtNLM2 source_version: UniProt 2024_06 pilot predicted_term: id: GO:0007338 label: single fertilization predicted_term_type: GO_BP review: assessment: NPI error_type: DOMAIN_ARCHITECTURE_MISMATCH confidence_score: 0 summary: >- The target sequence is a 156-residue single-CUB ORF from the fragmented 2017 bullfrog assembly, not a complete CUB-only paralog whose subfamily should be read as independent evidence for loss of ovochymase activity. The existing curated review and its OpenScientist core-function audit found that adjacent KV928989 ORFs reconstruct a tolloid-like CUB-EGF-CUB-CUB-EGF-CUB region and that the 2024 chromosome-level assembly encodes complete BMP-1 and TLL1 metalloproteases, so this accession is best treated as a fragment of an extracellular tolloid-family protease. The fertilization prediction is still incorrect for the sequence supplied to ProtNLM2: the ovochymase audit shows the short accession lacks any S1 serine-protease domain and the His-Asp-Ser triad required for the egg-envelope protease activity underlying GO:0007338. This is therefore a gene-model/input-sequence failure rather than paralogous transfer to a genuine stand-alone CUB protein. supported_by: - reference_id: file:AQUCT/A0A2G9RZF1/A0A2G9RZF1-uniprot.txt supporting_text: >- ID A0A2G9RZF1_AQUCT Unreviewed; 156 AA. ... DR InterPro; IPR000859; CUB_dom. ... DR PANTHER; PTHR24251:SF37; CUB DOMAIN-CONTAINING PROTEIN; 1. ... FT DOMAIN 31..147 ... FT /note="CUB" - reference_id: PMID:8510165 supporting_text: >- 31 copies of an extracellular domain, here called CUB - reference_id: file:AQUCT/A0A2G9RZF1/A0A2G9RZF1-hypotheses/prediction-ovochymase-fertilization/openscientist.md supporting_text: >- with no serine protease domain whatsoever and no catalytic triad. - reference_id: file:AQUCT/A0A2G9RZF1/A0A2G9RZF1-hypotheses/core-function-1-go-0005201/openscientist.md supporting_text: >- The three fragmented ORFs on scaffold KV928989 (156 + 228 + 250 = 634 aa combined) represent portions of one of these ~1,000 aa genes, with the N-terminal protease domain and additional domains falling in assembly gaps. - reference_id: file:AQUCT/A0A2G9RZF1/A0A2G9RZF1-ai-review.yaml supporting_text: >- A0A2G9RZF1 is a gene prediction fragment from the fragmented draft genome assembly of Aquarana catesbeiana - source_method: ProtNLM2 source_version: UniProt 2024_06 pilot predicted_term: id: GO:0005576 label: extracellular region predicted_term_type: GO_CC review: assessment: UNC confidence_score: 1 summary: >- The sequence contains a nearly complete CUB domain at residues 31-147, and the comparative analysis defining CUB domains places this module in extracellular proteins with diverse functions. The existing curated review also accepts extracellular region for the draft fragment because a CUB domain from a tolloid-family protease is expected to act extracellularly. The focused ovochymase audit nevertheless found no signal-peptide confirmation for the exact 156-residue UniProt entry, and the TrEMBL record has no FT SIGNAL feature. The broad extracellular-region prediction is therefore plausible and aligned with the main review's location inference, but still UNC for the exact short accession rather than confirmed from an experimentally supported mature product. supported_by: - reference_id: file:AQUCT/A0A2G9RZF1/A0A2G9RZF1-uniprot.txt supporting_text: >- ID A0A2G9RZF1_AQUCT Unreviewed; 156 AA. ... DR InterPro; IPR000859; CUB_dom. ... DR PANTHER; PTHR24251:SF37; CUB DOMAIN-CONTAINING PROTEIN; 1. ... FT DOMAIN 31..147 ... FT /note="CUB" - reference_id: PMID:8510165 supporting_text: >- 31 copies of an extracellular domain, here called CUB - reference_id: file:AQUCT/A0A2G9RZF1/A0A2G9RZF1-hypotheses/prediction-ovochymase-fertilization/openscientist.md supporting_text: >- an extracellular-region CC term could be a *lead* if a signal peptide is confirmed, but this was not tested and should not be asserted - reference_id: file:AQUCT/A0A2G9RZF1/A0A2G9RZF1-ai-review.yaml supporting_text: >- The fragment contains a single CUB domain (residues 31-147) that mediates protein-protein interactions in the extracellular space. references: - id: file:AQUCT/A0A2G9RZF1/A0A2G9RZF1-uniprot.txt title: A0A2G9RZF1-uniprot.txt - id: PMID:8510165 title: The CUB domain. A widespread module in developmentally regulated proteins. - id: file:AQUCT/A0A2G9RZF1/A0A2G9RZF1-ai-review.yaml title: A0A2G9RZF1 AI review - id: file:AQUCT/A0A2G9RZF1/A0A2G9RZF1-hypotheses/core-function-1-go-0005201/openscientist.md title: OpenScientist structural-constituent audit for A0A2G9RZF1 publication_type: DEEP_RESEARCH - id: file:AQUCT/A0A2G9RZF1/A0A2G9RZF1-hypotheses/prediction-ovochymase-fertilization/openscientist.md title: OpenScientist ovochymase/fertilization audit for A0A2G9RZF1 publication_type: DEEP_RESEARCH