ABF2

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0000976 transcription cis-regulatory region binding	IEA GO_REF:0000117	MODIFY	Summary: ABF2 binds ABRE-containing promoters with sequence specificity. Direct EMSA/ChIP and transactivation of NYE1 support the specific activity; the Y1H network paper supplies additional targets without making the same DNA-binding function non-core. Reason: ABF2 binds ABRE-containing promoters with sequence specificity. Direct EMSA/ChIP and transactivation of NYE1 support the specific activity; the Y1H network paper supplies additional targets without making the same DNA-binding function non-core. Proposed replacements: cis-regulatory region sequence-specific DNA binding Supporting Evidence: PMID:27373216 ABF2, ABF3, and ABF4 directly bound to and activated the NYE1 promoter in vitro and in vivo.
GO:0000976 transcription cis-regulatory region binding	IPI PMID:25533953 An Arabidopsis gene regulatory network for secondary cell wa...	MODIFY	Summary: ABF2 binds ABRE-containing promoters with sequence specificity. Direct EMSA/ChIP and transactivation of NYE1 support the specific activity; the Y1H network paper supplies additional targets without making the same DNA-binding function non-core. Reason: ABF2 binds ABRE-containing promoters with sequence specificity. Direct EMSA/ChIP and transactivation of NYE1 support the specific activity; the Y1H network paper supplies additional targets without making the same DNA-binding function non-core. Proposed replacements: cis-regulatory region sequence-specific DNA binding Supporting Evidence: PMID:27373216 ABF2, ABF3, and ABF4 directly bound to and activated the NYE1 promoter in vitro and in vivo.
GO:0000976 transcription cis-regulatory region binding	IPI PMID:27373216 ABF2, ABF3, and ABF4 Promote ABA-Mediated Chlorophyll Degrad...	MODIFY	Summary: ABF2 binds ABRE-containing promoters with sequence specificity. Direct EMSA/ChIP and transactivation of NYE1 support the specific activity; the Y1H network paper supplies additional targets without making the same DNA-binding function non-core. Reason: ABF2 binds ABRE-containing promoters with sequence specificity. Direct EMSA/ChIP and transactivation of NYE1 support the specific activity; the Y1H network paper supplies additional targets without making the same DNA-binding function non-core. Proposed replacements: cis-regulatory region sequence-specific DNA binding Supporting Evidence: PMID:27373216 ABF2, ABF3, and ABF4 directly bound to and activated the NYE1 promoter in vitro and in vivo.
GO:0003700 DNA-binding transcription factor activity	IEA GO_REF:0000002	ACCEPT	Summary: ABF2/AREB1 is a DNA-binding transcriptional activator. Reason: bZIP classification is independently supported by direct ABRE-dependent reporter transactivation and target-promoter binding. Supporting Evidence: PMID:11005831 both the AREB1 and AREB2 proteins activated transcription of a reporter gene driven by ABRE. PMID:27373216 ABF2, ABF3, and ABF4 directly bound to and activated the NYE1 promoter in vitro and in vivo.
GO:0003700 DNA-binding transcription factor activity	ISS PMID:11906833 bZIP transcription factors in Arabidopsis.	ACCEPT	Summary: ABF2/AREB1 is a DNA-binding transcriptional activator. Reason: bZIP classification is independently supported by direct ABRE-dependent reporter transactivation and target-promoter binding. Supporting Evidence: PMID:11005831 both the AREB1 and AREB2 proteins activated transcription of a reporter gene driven by ABRE. PMID:27373216 ABF2, ABF3, and ABF4 directly bound to and activated the NYE1 promoter in vitro and in vivo.
GO:0005515 protein binding	IPI PMID:15516505 ARIA, an Arabidopsis arm repeat protein interacting with a t...	REMOVE	Summary: The interaction is regulatory context for the ABF2 transcription factor. Reason: ARIA directly interacts with ABF2 and modulates its transcriptional function. Remove generic protein binding as uninformative; the partner association is not disputed. Supporting Evidence: PMID:15516505
GO:0005515 protein binding	IPI PMID:16299177 Arabidopsis calcium-dependent protein kinase AtCPK32 interac...	REMOVE	Summary: The interaction is regulatory context for the ABF2 transcription factor. Reason: The abstract emphasizes CPK32-ABF4, and the ABF2-specific interaction cannot be resolved from the available full-text access. Do not infer a wrong-gene error. Remove only the uninformative generic protein-binding term; kinase regulation of ABF2 is contextual, not an ABF2 kinase activity. Supporting Evidence: PMID:16299177
GO:0005515 protein binding	IPI PMID:19924127 In vitro reconstitution of an abscisic acid signalling pathw...	REMOVE	Summary: The interaction is regulatory context for the ABF2 transcription factor. Reason: The reconstitution directly identifies ABF2 as the OST1 phosphorylation substrate and transcriptional effector. This does not assign protein kinase activity to ABF2; bare binding is uninformative. Supporting Evidence: PMID:19924127
GO:0005515 protein binding	IPI PMID:32167237 Trehalose-6-phosphate phosphatase E modulates ABA-controlled...	MODIFY	Summary: The full paper identifies the interaction with the TPPE promoter through Y1H, EMSA, ChIP and promoter reporters. This supports ABRE-specific DNA binding, not a demonstrated ABF2-TPPE protein interaction. Correct the interaction type while preserving the experimentally supported regulatory relationship. Reason: The full paper identifies the interaction with the TPPE promoter through Y1H, EMSA, ChIP and promoter reporters. This supports ABRE-specific DNA binding, not a demonstrated ABF2-TPPE protein interaction. Correct the interaction type while preserving the experimentally supported regulatory relationship. Proposed replacements: cis-regulatory region sequence-specific DNA binding Supporting Evidence: PMID:32167237 directly binds to the TPPE promoter to activate its expression.
GO:0005634 nucleus	IBA GO_REF:0000033	ACCEPT	Summary: ABF2/AREB1 is nuclear. Reason: Direct fluorescent-fusion localization in PMID:15516505 and PMID:16284313 supports the nuclear site of transcriptional activity, independently corroborating the IBA and sequence predictions. Supporting Evidence: PMID:15516505 ABF2 is a transcription factor and, as shown in Figure 2C (top section), is localized in the nucleus.
GO:0005634 nucleus	IDA PMID:15516505 ARIA, an Arabidopsis arm repeat protein interacting with a t...	ACCEPT	Summary: ABF2/AREB1 is nuclear. Reason: Direct fluorescent-fusion localization in PMID:15516505 and PMID:16284313 supports the nuclear site of transcriptional activity, independently corroborating the IBA and sequence predictions. Supporting Evidence: PMID:15516505 ABF2 is a transcription factor and, as shown in Figure 2C (top section), is localized in the nucleus.
GO:0005634 nucleus	IDA PMID:16284313 AREB1 is a transcription activator of novel ABRE-dependent A...	ACCEPT	Summary: ABF2/AREB1 is nuclear. Reason: Direct fluorescent-fusion localization in PMID:15516505 and PMID:16284313 supports the nuclear site of transcriptional activity, independently corroborating the IBA and sequence predictions. Supporting Evidence: PMID:15516505 ABF2 is a transcription factor and, as shown in Figure 2C (top section), is localized in the nucleus.
GO:0005634 nucleus	IEA GO_REF:0000044	ACCEPT	Summary: ABF2/AREB1 is nuclear. Reason: Direct fluorescent-fusion localization in PMID:15516505 and PMID:16284313 supports the nuclear site of transcriptional activity, independently corroborating the IBA and sequence predictions. Supporting Evidence: PMID:15516505 ABF2 is a transcription factor and, as shown in Figure 2C (top section), is localized in the nucleus.
GO:0005634 nucleus	ISM GO_REF:0000122	ACCEPT	Summary: ABF2/AREB1 is nuclear. Reason: Direct fluorescent-fusion localization in PMID:15516505 and PMID:16284313 supports the nuclear site of transcriptional activity, independently corroborating the IBA and sequence predictions. Supporting Evidence: PMID:15516505 ABF2 is a transcription factor and, as shown in Figure 2C (top section), is localized in the nucleus.
GO:0006355 regulation of DNA-templated transcription	IEA GO_REF:0000002	MODIFY	Summary: Direct ABRE-driven reporter activation supports positive transcriptional regulation, the more specific process already experimentally annotated. Reason: Direct ABRE-driven reporter activation supports positive transcriptional regulation, the more specific process already experimentally annotated. Proposed replacements: positive regulation of DNA-templated transcription Supporting Evidence: PMID:11005831 both the AREB1 and AREB2 proteins activated transcription of a reporter gene driven by ABRE.
GO:0009414 response to water deprivation	IEA GO_REF:0000117	ACCEPT	Summary: ABF2 participates in ABA-responsive transcription during vegetative drought responses. Reason: Induction and transactivation are supported by primary studies; activated AREB1 enhances drought tolerance and a dominant repressor reduces dehydration survival. These results support the process without claiming that a single abf2 null always has a drought defect: PMID:15361142 reported no such single-mutant defect in its conditions. Supporting Evidence: PMID:16284313 AREB1DeltaQT-overexpressing plants showed ABA hypersensitivity and enhanced drought tolerance PMID:15361142 these mutants displayed defects in ABA, salt, and dehydration responses, which were not observed with the abf2 mutant.
GO:0009414 response to water deprivation	IEP PMID:16284313 AREB1 is a transcription activator of novel ABRE-dependent A...	ACCEPT	Summary: ABF2 participates in ABA-responsive transcription during vegetative drought responses. Reason: Induction and transactivation are supported by primary studies; activated AREB1 enhances drought tolerance and a dominant repressor reduces dehydration survival. These results support the process without claiming that a single abf2 null always has a drought defect: PMID:15361142 reported no such single-mutant defect in its conditions. Supporting Evidence: PMID:16284313 AREB1DeltaQT-overexpressing plants showed ABA hypersensitivity and enhanced drought tolerance PMID:15361142 these mutants displayed defects in ABA, salt, and dehydration responses, which were not observed with the abf2 mutant.
GO:0009414 response to water deprivation	IEP PMID:16463099 Transcriptional regulation of ABI3- and ABA-responsive genes...	ACCEPT	Summary: ABF2 participates in ABA-responsive transcription during vegetative drought responses. Reason: Induction and transactivation are supported by primary studies; activated AREB1 enhances drought tolerance and a dominant repressor reduces dehydration survival. These results support the process without claiming that a single abf2 null always has a drought defect: PMID:15361142 reported no such single-mutant defect in its conditions. Supporting Evidence: PMID:16284313 AREB1DeltaQT-overexpressing plants showed ABA hypersensitivity and enhanced drought tolerance PMID:15361142 these mutants displayed defects in ABA, salt, and dehydration responses, which were not observed with the abf2 mutant.
GO:0009414 response to water deprivation	IMP PMID:16284313 AREB1 is a transcription activator of novel ABRE-dependent A...	ACCEPT	Summary: ABF2 participates in ABA-responsive transcription during vegetative drought responses. Reason: Induction and transactivation are supported by primary studies; activated AREB1 enhances drought tolerance and a dominant repressor reduces dehydration survival. These results support the process without claiming that a single abf2 null always has a drought defect: PMID:15361142 reported no such single-mutant defect in its conditions. Supporting Evidence: PMID:16284313 AREB1DeltaQT-overexpressing plants showed ABA hypersensitivity and enhanced drought tolerance PMID:15361142 these mutants displayed defects in ABA, salt, and dehydration responses, which were not observed with the abf2 mutant.
GO:0009651 response to salt stress	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: Salt stress induces AREB1/ABF2 in the broader ABA stress-response program. Reason: Salt-responsive expression and stress-tolerance phenotypes support this specific environmental response, retained as secondary to ABRE-dependent ABA/drought transcription. Expression induction alone does not establish a salt-specific biochemical activity or obligatory single-mutant phenotype. Supporting Evidence: PMID:11005831 Transcription of the AREB1 and AREB2 genes is up-regulated by drought, NaCl, and ABA treatment in vegetative tissues.
GO:0009651 response to salt stress	IEP PMID:16284313 AREB1 is a transcription activator of novel ABRE-dependent A...	KEEP AS NON CORE	Summary: Salt stress induces AREB1/ABF2 in the broader ABA stress-response program. Reason: Salt-responsive expression and stress-tolerance phenotypes support this specific environmental response, retained as secondary to ABRE-dependent ABA/drought transcription. Expression induction alone does not establish a salt-specific biochemical activity or obligatory single-mutant phenotype. Supporting Evidence: PMID:11005831 Transcription of the AREB1 and AREB2 genes is up-regulated by drought, NaCl, and ABA treatment in vegetative tissues.
GO:0009651 response to salt stress	IEP PMID:16463099 Transcriptional regulation of ABI3- and ABA-responsive genes...	KEEP AS NON CORE	Summary: Salt stress induces AREB1/ABF2 in the broader ABA stress-response program. Reason: Salt-responsive expression and stress-tolerance phenotypes support this specific environmental response, retained as secondary to ABRE-dependent ABA/drought transcription. Expression induction alone does not establish a salt-specific biochemical activity or obligatory single-mutant phenotype. Supporting Evidence: PMID:11005831 Transcription of the AREB1 and AREB2 genes is up-regulated by drought, NaCl, and ABA treatment in vegetative tissues.
GO:0009737 response to abscisic acid	IEA GO_REF:0000117	ACCEPT	Summary: ABF2 is an ABA-activated transcriptional effector. Reason: ABA-dependent promoter activation and phosphorylation establish direct participation in the hormone response, beyond simple induction of ABF2 expression. Supporting Evidence: PMID:11005831 AREB1 and AREB2 required ABA for their activation PMID:11005831 both the AREB1 and AREB2 proteins activated transcription of a reporter gene driven by ABRE.
GO:0009737 response to abscisic acid	IEP PMID:16284313 AREB1 is a transcription activator of novel ABRE-dependent A...	ACCEPT	Summary: ABF2 is an ABA-activated transcriptional effector. Reason: ABA-dependent promoter activation and phosphorylation establish direct participation in the hormone response, beyond simple induction of ABF2 expression. Supporting Evidence: PMID:11005831 AREB1 and AREB2 required ABA for their activation PMID:11005831 both the AREB1 and AREB2 proteins activated transcription of a reporter gene driven by ABRE.
GO:0009737 response to abscisic acid	IEP PMID:16463099 Transcriptional regulation of ABI3- and ABA-responsive genes...	ACCEPT	Summary: ABF2 is an ABA-activated transcriptional effector. Reason: ABA-dependent promoter activation and phosphorylation establish direct participation in the hormone response, beyond simple induction of ABF2 expression. Supporting Evidence: PMID:11005831 AREB1 and AREB2 required ABA for their activation PMID:11005831 both the AREB1 and AREB2 proteins activated transcription of a reporter gene driven by ABRE.
GO:0010255 glucose mediated signaling pathway	IMP PMID:15361142 ABF2, an ABRE-binding bZIP factor, is an essential component...	KEEP AS NON CORE	Summary: ABF2 contributes to glucose-dependent developmental responses. Reason: ABF2 overexpression enhances glucose-mediated growth inhibition and abf2 mutation impairs glucose response. This supported role is distinct from the central ABA-responsive transcriptional mechanism and does not imply direct glucose binding. Supporting Evidence: PMID:15361142 ABF2 overexpression promoted glucose-induced inhibition of seedling development, whereas its mutation impaired glucose response.
GO:0045893 positive regulation of DNA-templated transcription	IDA PMID:11005831 Arabidopsis basic leucine zipper transcription factors invol...	ACCEPT	Summary: ABF2 activates transcription of ABRE-containing target genes. Reason: Direct AREB1/ABF2 transactivation in PMID:11005831 and PMID:16463099 establishes the core activity. The source PMID:19207209 emphasizes bZIP67 in its abstract; its ABF2-specific experiment is not independently resolved here, and that limitation does not overturn a directly corroborated experimental claim. Supporting Evidence: PMID:11005831 both the AREB1 and AREB2 proteins activated transcription of a reporter gene driven by ABRE. PMID:16463099 ABI3, ABI5 and AREB1 activated transcription of a GUS reporter gene driven by the RD29B promoter strongly
GO:0045893 positive regulation of DNA-templated transcription	IDA PMID:16463099 Transcriptional regulation of ABI3- and ABA-responsive genes...	ACCEPT	Summary: ABF2 activates transcription of ABRE-containing target genes. Reason: Direct AREB1/ABF2 transactivation in PMID:11005831 and PMID:16463099 establishes the core activity. The source PMID:19207209 emphasizes bZIP67 in its abstract; its ABF2-specific experiment is not independently resolved here, and that limitation does not overturn a directly corroborated experimental claim. Supporting Evidence: PMID:11005831 both the AREB1 and AREB2 proteins activated transcription of a reporter gene driven by ABRE. PMID:16463099 ABI3, ABI5 and AREB1 activated transcription of a GUS reporter gene driven by the RD29B promoter strongly
GO:0045893 positive regulation of DNA-templated transcription	IDA PMID:19207209 Arabidopsis NF-YB subunits LEC1 and LEC1-LIKE activate trans...	ACCEPT	Summary: ABF2 activates transcription of ABRE-containing target genes. Reason: Direct AREB1/ABF2 transactivation in PMID:11005831 and PMID:16463099 establishes the core activity. The source PMID:19207209 emphasizes bZIP67 in its abstract; its ABF2-specific experiment is not independently resolved here, and that limitation does not overturn a directly corroborated experimental claim. Supporting Evidence: PMID:11005831 both the AREB1 and AREB2 proteins activated transcription of a reporter gene driven by ABRE. PMID:16463099 ABI3, ABI5 and AREB1 activated transcription of a GUS reporter gene driven by the RD29B promoter strongly
GO:0045893 positive regulation of DNA-templated transcription	IEA GO_REF:0000120	ACCEPT	Summary: ABF2 activates transcription of ABRE-containing target genes. Reason: Direct AREB1/ABF2 transactivation in PMID:11005831 and PMID:16463099 establishes the core activity. The source PMID:19207209 emphasizes bZIP67 in its abstract; its ABF2-specific experiment is not independently resolved here, and that limitation does not overturn a directly corroborated experimental claim. Supporting Evidence: PMID:11005831 both the AREB1 and AREB2 proteins activated transcription of a reporter gene driven by ABRE. PMID:16463099 ABI3, ABI5 and AREB1 activated transcription of a GUS reporter gene driven by the RD29B promoter strongly
GO:1901701 cellular response to oxygen-containing compound	IEA GO_REF:0000117	MODIFY	Summary: ABF2 executes ABA-dependent changes in cellular gene expression. Refine the broad oxygen-containing-compound response to the demonstrated cellular ABA response. Reason: ABF2 executes ABA-dependent changes in cellular gene expression. Refine the broad oxygen-containing-compound response to the demonstrated cellular ABA response. Proposed replacements: cellular response to abscisic acid stimulus Supporting Evidence: PMID:11005831 AREB1 and AREB2 required ABA for their activation PMID:11005831 both the AREB1 and AREB2 proteins activated transcription of a reporter gene driven by ABRE.

Gene Description

Existing Annotations Review

Core Functions

Nuclear ABRE-binding bZIP transcription factor whose ABA-dependent phosphorylation activates target gene expression in vegetative drought responses. Overlapping ABF/AREB activities and activation-state dependence explain why single-mutant and overexpression phenotypes differ.

References

📚 Additional Documentation

Notes

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