ABI5

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0003700 DNA-binding transcription factor activity	IEA GO_REF:0000002	ACCEPT	Summary: ABI5 is a bZIP transcription factor that binds ABRE cis-elements and activates ABA/seed gene expression. DNA-binding transcription factor activity is the core molecular function, well supported experimentally. Reason: Core molecular function. Established by cloning of ABI5 as a bZIP TF whose DNA-binding/dimerization domains are required for function, and by direct promoter binding and transactivation assays. Supporting Evidence: PMID:10760247 it encodes a member of the basic leucine zipper transcription factor family. The previously characterized abi5-1 allele encodes a protein that lacks the DNA binding and dimerization domains required for ABI5 function file:ARATH/ABI5/ABI5-deep-research-falcon.md ABI5 is a basic leucine zipper (bZIP) transcription factor that binds ACGT-core cis-elements (e.g., G-box and ABRE-related motifs) to regulate ABA/stress-responsive gene expression, especially in seeds and early seedlings.
GO:0005634 nucleus	IEA GO_REF:0000120	ACCEPT	Summary: ABI5 is a nuclear transcription factor. GFP fusions of ABI5-family proteins localize constitutively to the nucleus, and UniProt curates a nuclear location. Reason: Correct subcellular location for a transcription factor, consistent with experimental localization and the bZIP nuclear localization signal. Supporting Evidence: PMID:15642716 the three fusion proteins were expressed with a largely overlapping pattern and constitutively localized in the nuclei file:ARATH/ABI5/ABI5-deep-research-falcon.md ABI5 functions in the nucleus.
GO:0006355 regulation of DNA-templated transcription	IEA GO_REF:0000120	ACCEPT	Summary: ABI5 regulates transcription of ABA-responsive genes. This is a broad but correct process term for a transcription factor. Reason: Accurate, if general, process annotation. More specific positive regulation is also annotated (GO:0045893). Supporting Evidence: PMID:10760247 ABI5 regulates a subset of late embryogenesis-abundant genes during both developmental stages file:ARATH/ABI5/ABI5-deep-research-falcon.md ABI5 is a sequence-specific transcription factor (bZIP/AREB/ABF family) that binds ABRE/ACGT-core motifs and modulates transcription of ABA-responsive genes, functioning as a central transcriptional effector downstream of SnRK2 kinases.
GO:0007165 signal transduction	IEA GO_REF:0000117	MARK AS OVER ANNOTATED	Summary: ABI5 acts within the ABA signaling pathway, but the generic 'signal transduction' term is too broad and uninformative; the specific ABA-activated signaling pathway is the appropriate term. Reason: ABI5 is a downstream transcriptional effector of ABA signaling rather than a general signal transducer. The generic parent over-annotates; the more specific abscisic acid-activated signaling pathway (a UniProt keyword annotation) captures this better. Supporting Evidence: PMID:11287670 ABI5 protein accumulation, phosphorylation, stability, and activity are highly regulated by ABA during germination and early seedling growth
GO:0009414 response to water deprivation	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: ABI5 participates in drought/osmotic responses; its expression is induced by water stress and it activates osmotolerance (LEA) genes. Duplicate of the experimentally supported IEP annotation. Reason: Supported but peripheral relative to the core seed/ABA transcriptional role; ABI5 acts within the drought response rather than this being its defining function. Supporting Evidence: PMID:11287670 ABI5 abundance closely reflects environmental conditions during the 60 h following stratification. Within this time period, ABI5 levels increase on imposition of water stress
GO:0009651 response to salt stress	IEA GO_REF:0000117	KEEP AS NON CORE	Summary: ABI5 expression and activity respond to salt/osmotic stress; duplicate of the experimentally supported IEP annotation. Reason: Supported but a peripheral stress-response role downstream of ABA, not the core seed transcriptional function. Supporting Evidence: PMID:11287670 ABI5 expression is regulated by drought and salt exposure
GO:0009737 response to abscisic acid	IEA GO_REF:0000117	ACCEPT	Summary: ABA response is central to ABI5 biology; ABI5 is the major mediator of ABA repression of growth and ABA-regulated gene expression. Duplicate of experimentally supported annotations. Reason: Core biological context. ABI5 is induced by and acts within the ABA response. Supporting Evidence: PMID:11287670 ABI5 protein accumulation, phosphorylation, stability, and activity are highly regulated by ABA during germination and early seedling growth file:ARATH/ABI5/ABI5-deep-research-falcon.md ABI5 (Q9SJN0; At2g36270) is best annotated as a nuclear bZIP transcription factor that integrates ABA core signaling into transcriptional programs that suppress germination and early seedling growth under stress.
GO:0009845 seed germination	IEA GO_REF:0000117	ACCEPT	Summary: ABI5 controls germination, acting as a repressor of germination downstream of ABA/GA. Duplicate of experimentally supported IEP annotation. Reason: Core process. The more specific negative regulation of seed germination (GO:0010187) is also annotated experimentally. Supporting Evidence: PMID:18941053 ABI5 acts as the final common repressor of germination in response to changes in ABA and GA levels file:ARATH/ABI5/ABI5-deep-research-falcon.md Multiple recent studies converge on ABI5 as a master regulator of ABA-mediated growth arrest during the embryo-to-seedling transition, repressing suites of growth-related genes (photosynthesis and cell-wall organization categories) when ABA is elevated.
GO:0043565 sequence-specific DNA binding	IEA GO_REF:0000117	ACCEPT	Summary: ABI5 binds the ABRE sequence in target promoters in a sequence-specific manner via its bZIP basic region. Well supported. Reason: Correct, informative molecular function for a bZIP ABRE-binding TF; direct promoter binding demonstrated experimentally. Supporting Evidence: PMID:11489176 only ABI5 binds directly to this promoter fragment file:ARATH/ABI5/ABI5-deep-research-falcon.md ABREs are commonly described as cis-elements with a conserved core such as (C/T)ACGTGGC, and ABI5/ABF proteins bind ABREs and related ACGT-core elements (e.g., G-box CACGTG).
GO:0045893 positive regulation of DNA-templated transcription	IEA GO_REF:0000002	ACCEPT	Summary: ABI5 is a transcriptional activator of ABA-responsive/LEA genes. Duplicate of experimentally supported IDA annotations. Reason: Core function. ABI5 activates transcription of ABRE-containing target genes such as RD29B and Em1/Em6. Supporting Evidence: PMID:16463099 ABI3, ABI5 and AREB1 activated transcription of a GUS reporter gene driven by the RD29B promoter strongly file:ARATH/ABI5/ABI5-deep-research-falcon.md ABI5 co-target genes with ABI3/ABI4 and classical ABA marker genes include EM1, EM6, RD29B, RAB18 and LEA genes
GO:1901701 cellular response to oxygen-containing compound	IEA GO_REF:0000117	MARK AS OVER ANNOTATED	Summary: This very broad term is an automated ARBA mapping (abscisic acid is an oxygen-containing compound). It is subsumed by the specific response to abscisic acid annotation and adds no information. Reason: Uninformative high-level grouping term derived automatically; the specific response to abscisic acid term (GO:0009737) is the appropriate annotation. Supporting Evidence: PMID:11287670 ABI5 protein accumulation, phosphorylation, stability, and activity are highly regulated by ABA during germination and early seedling growth
GO:0005515 protein binding	IPI PMID:17194765 KEEP ON GOING, a RING E3 ligase essential for Arabidopsis gr...	REMOVE	Summary: Interaction with the RING E3 ligase KEG, which mediates ABA-dependent ABI5 degradation. This is a regulatory interaction; the bare 'protein binding' term is uninformative about ABI5 molecular function. Reason: Generic protein binding is discouraged as a core molecular function. The biologically meaningful function (DNA-binding TF activity) is captured by other terms; this interaction (KEG regulating ABI5 stability) is recorded in the notes/description. Supporting Evidence: PMID:17194765 KEEP ON GOING, a RING E3 ligase essential for Arabidopsis growth and development, is involved in abscisic acid signaling
GO:0005515 protein binding	IPI PMID:19276109 Sumoylation of ABI5 by the Arabidopsis SUMO E3 ligase SIZ1 n...	REMOVE	Summary: Interaction with SUMO1 (sumoylation of ABI5 by SIZ1 at Lys-391). A post-translational modification interaction; the generic protein binding term is uninformative. Reason: Bare protein binding is not an informative molecular function. The sumoylation regulation is documented in the notes and as a UniProt PTM. Supporting Evidence: PMID:19276109 Sumoylation of ABI5 by the Arabidopsis SUMO E3 ligase SIZ1 negatively regulates abscisic acid signaling
GO:0005515 protein binding	IPI PMID:32612234 Extensive signal integration by the phytohormone protein net...	REMOVE	Summary: High-throughput phytohormone protein interaction network (interactions with SnRK2 kinase SRK2A and IAA30). Generic protein binding term, not informative about ABI5 function. Reason: Uninformative bare protein binding from an interactome screen; ABI5's informative MF is DNA-binding TF activity. Supporting Evidence: PMID:32612234 Extensive signal integration by the phytohormone protein network
GO:0005515 protein binding	IPI PMID:40123384 Nitrate attenuates abscisic acid signaling via NIN-LIKE PROT...	REMOVE	Summary: Interaction with NLP8 in the nitrate/ABA crosstalk controlling seed germination. Generic protein binding term, uninformative about ABI5 molecular function. Reason: Bare protein binding is discouraged as a core molecular function; the relevant ABI5 MF is captured by DNA-binding TF activity terms. Supporting Evidence: PMID:40123384 Nitrate attenuates abscisic acid signaling via NIN-LIKE PROTEIN8 in Arabidopsis seed germination
GO:0000987 cis-regulatory region sequence-specific DNA binding	IPI PMID:27095403 The LEA protein, ABR, is regulated by ABI5 and involved in d...	ACCEPT	Summary: ABI5 binds the promoter (ABRE/cis-regulatory region) of the LEA gene ABR in vitro and in vivo, directly regulating its expression. Informative DNA-binding molecular function. Reason: Supported, specific DNA-binding MF consistent with ABI5 binding ABRE cis-elements in target gene promoters. Supporting Evidence: PMID:27095403 In vitro and in vivo assays showed that ABI5 bind to the ABR promoter, indicating that ABI5 directly regulates the expression of ABR file:ARATH/ABI5/ABI5-deep-research-falcon.md ABI5 enrichment at promoters (e.g., GUN5, PSAH1, PBSR, PBSQ2, XTH7) decreased when FLZ13 was knocked down, supporting FLZ13 as a cofactor that enhances ABI5 occupancy at target loci.
GO:0000976 transcription cis-regulatory region binding	IPI PMID:25533953 An Arabidopsis gene regulatory network for secondary cell wa...	KEEP AS NON CORE	Summary: ABI5 was detected binding secondary cell wall gene promoters in a large high-throughput yeast one-hybrid network screen. The generic cis-regulatory binding MF is acceptable, but the biological inference that ABI5 regulates cell-wall genes is weak (Y1H over-detection for a seed-predominant TF). Reason: The DNA-binding MF itself is consistent with ABI5 being a bZIP, but this interaction comes from a genome-scale Y1H screen unrelated to ABI5's established seed/ABA biology; retained as non-core. Supporting Evidence: PMID:25533953 We therefore chose to pursue a network-based approach to comprehensively characterize the transcriptional regulation of secondary cell wall biosynthesis
GO:0000976 transcription cis-regulatory region binding	IPI PMID:30356219 Transcriptional regulation of nitrogen-associated metabolism...	KEEP AS NON CORE	Summary: ABI5 detected binding promoters in a genome-scale yeast one-hybrid network for nitrogen-associated metabolism. Generic DNA-binding MF acceptable; biological relevance to ABI5 function is uncertain. Reason: High-throughput Y1H-derived interaction outside ABI5's characterized seed/ABA role; the DNA-binding MF is plausible but non-core. Supporting Evidence: PMID:30356219 we present a transcriptional regulatory network and twenty-one transcription factors that regulate the architecture of root and shoot systems in response to changes in nitrogen availability
GO:0005515 protein binding	IPI PMID:23404889 The PP6 phosphatase regulates ABI5 phosphorylation and absci...	REMOVE	Summary: Interaction with PP6/FYPP-type phosphatase subunits regulating ABI5 phosphorylation. Regulatory interaction captured by generic, uninformative protein binding term. Reason: Bare protein binding is discouraged; the phosphatase regulation of ABI5 is recorded in the notes/description. Supporting Evidence: PMID:23404889 The PP6 phosphatase regulates ABI5 phosphorylation and abscisic acid signaling in Arabidopsis
GO:0000976 transcription cis-regulatory region binding	IPI PMID:31806676 A PXY-Mediated Transcriptional Network Integrates Signaling ...	KEEP AS NON CORE	Summary: ABI5 detected binding vascular-development gene promoters in a genome-scale yeast one-hybrid (eY1H) network. Generic DNA-binding MF is acceptable, but the inference of an ABI5 role in vascular development is weak. Reason: High-throughput Y1H network interaction unrelated to ABI5's established seed/ABA biology; DNA-binding MF plausible but non-core. Supporting Evidence: PMID:31806676 we mapped a putative PXY-mediated transcriptional regulatory network comprising 690 transcription factor-promoter interactions in Arabidopsis
GO:0005515 protein binding	IPI PMID:31295628 Nucleocytoplasmic Trafficking of the Arabidopsis WD40 Repeat...	REMOVE	Summary: Interaction with the WD40 protein XIW1, which regulates ABI5 stability and ABA responses. Regulatory interaction with an uninformative protein binding term. Reason: Bare protein binding is discouraged as a core molecular function; the XIW1-mediated regulation of ABI5 stability is documented in notes. Supporting Evidence: PMID:31295628 Nucleocytoplasmic Trafficking of the Arabidopsis WD40 Repeat Protein XIW1 Regulates ABI5 Stability and Abscisic Acid Responses
GO:0005515 protein binding	IPI PMID:29771466 Arabidopsis VQ18 and VQ26 proteins interact with ABI5 transc...	REMOVE	Summary: Interaction with VQ18 and VQ26 proteins that negatively modulate ABA response during germination via ABI5. Regulatory interaction; generic protein binding term is uninformative. Reason: Bare protein binding is discouraged; the VQ-protein modulation of ABI5 is recorded in the notes. Supporting Evidence: PMID:29771466 Arabidopsis VQ18 and VQ26 proteins interact with ABI5 transcription factor to negatively modulate ABA response during seed germination
GO:0005634 nucleus	ISM GO_REF:0000122	ACCEPT	Summary: Predicted nuclear localization (AtSubP). Consistent with experimental data and ABI5's role as a transcription factor. Reason: Correct location, in agreement with experimental localization (IDA) and the bZIP nuclear localization signal. Supporting Evidence: PMID:15642716 constitutively localized in the nuclei
GO:0005515 protein binding	IPI PMID:24357600 TAP46 plays a positive role in the ABSCISIC ACID INSENSITIVE...	REMOVE	Summary: Interaction with TAP46 (and PP2A), a positive regulator of ABI5-regulated gene expression. Regulatory interaction captured by an uninformative protein binding term. Reason: Bare protein binding is discouraged as a core molecular function; the TAP46/PP2A regulatory relationship is documented in notes/UniProt. Supporting Evidence: PMID:24357600 TAP46 plays a positive role in the ABSCISIC ACID INSENSITIVE5-regulated gene expression in Arabidopsis
GO:0045893 positive regulation of DNA-templated transcription	IDA PMID:19207209 Arabidopsis NF-YB subunits LEC1 and LEC1-LIKE activate trans...	ACCEPT	Summary: ABI5 acts as a seed-specific ABRE-binding factor that, with NF-Y (LEC1/L1L) partners, activates seed maturation gene transcription. Supports ABI5's role as a transcriptional activator. Reason: Core function. ABI5 positively regulates transcription of ABRE-driven seed/ABA target genes. Supporting Evidence: PMID:19207209 LEC1/L1L-[NF-YC2] can strongly activate the CRC promoter in the absence of ABA when co-expressed with a seed-specific ABA-response element (ABRE)-binding factor
GO:0045893 positive regulation of DNA-templated transcription	IDA PMID:16463099 Transcriptional regulation of ABI3- and ABA-responsive genes...	ACCEPT	Summary: ABI5 activates transcription of the ABRE-containing RD29B promoter in transient transactivation assays. Direct support for transcriptional activator function. Reason: Core function. Demonstrates ABI5-mediated transcriptional activation of a defined ABA-responsive target. Supporting Evidence: PMID:16463099 ABI3, ABI5 and AREB1 activated transcription of a GUS reporter gene driven by the RD29B promoter strongly file:ARATH/ABI5/ABI5-deep-research-falcon.md which then drive ABRE-centered transcriptional programs
GO:0005515 protein binding	IPI PMID:20525848 DWA1 and DWA2, two Arabidopsis DWD protein components of CUL...	REMOVE	Summary: Interaction with DWA1/DWA2 (CUL4-based E3 ligase DWD components) that negatively regulate ABA signaling. Regulatory degradation-related interaction; generic protein binding term is uninformative. Reason: Bare protein binding is discouraged; the DWA1/DWA2 regulation of ABI5 is documented in notes. Supporting Evidence: PMID:20525848 DWA1 and DWA2, two Arabidopsis DWD protein components of CUL4-based E3 ligases, act together as negative regulators in ABA signal transduction
GO:0006355 regulation of DNA-templated transcription	IMP PMID:10760247 The Arabidopsis abscisic acid response gene ABI5 encodes a b...	ACCEPT	Summary: abi5 mutants have altered expression of ABA-regulated genes, showing ABI5 regulates transcription. Broad but correct process term supported by mutant analysis. Reason: Accurate process annotation; the more specific positive regulation is also annotated. Supporting Evidence: PMID:10760247 ABI5 regulates a subset of late embryogenesis-abundant genes during both developmental stages
GO:0009737 response to abscisic acid	IEP PMID:18941053 The gibberellic acid signaling repressor RGL2 inhibits Arabi...	ACCEPT	Summary: ABI5 acts within the ABA response controlling germination; its induction and activity respond to ABA levels. Core ABA-response role. Reason: Supported by expression/genetic analysis linking ABI5 to ABA-controlled germination. Supporting Evidence: PMID:18941053 ABA blocks germination by inducing ABI5 (for ABA-INSENSITIVE5), a basic domain/leucine zipper transcription factor repressing germination
GO:0009739 response to gibberellin	IEP PMID:18941053 The gibberellic acid signaling repressor RGL2 inhibits Arabi...	KEEP AS NON CORE	Summary: ABI5 acts within the GA-ABA module controlling germination; low GA elevates ABA and ABI5, integrating the antagonistic hormones. A peripheral role relative to the core ABA-effector function. Reason: Supported, but reflects GA-ABA crosstalk at the germination checkpoint rather than a direct ABI5 GA-response function; non-core. Supporting Evidence: PMID:18941053 ABI5 acts as the final common repressor of germination in response to changes in ABA and GA levels
GO:0009845 seed germination	IEP PMID:18941053 The gibberellic acid signaling repressor RGL2 inhibits Arabi...	ACCEPT	Summary: ABI5 controls seed germination as a repressor downstream of ABA/GA. Core process; more specific negative regulation term is also annotated. Reason: Well supported. ABI5 is the major downstream player repressing germination of nondormant seeds. Supporting Evidence: PMID:18941053 ABI5, whose expression and product activity respond to changes in ABA and GA levels, is the main downstream player repressing the germination of nondormant seeds
GO:0010187 negative regulation of seed germination	IMP PMID:18941053 The gibberellic acid signaling repressor RGL2 inhibits Arabi...	ACCEPT	Summary: ABI5 represses seed germination; abi5/expression analyses show it is the final common repressor of germination in response to ABA and GA. Specific, core process function. Reason: Core biological process, strongly supported by genetic and expression evidence. Supporting Evidence: PMID:18941053 Increased ABI5 protein is ultimately responsible for preventing seed germination when GA levels are reduced file:ARATH/ABI5/ABI5-deep-research-falcon.md A 2024 study connects ABI5 to cell-wall remodeling during ABA-inhibited germination by showing ABI5 directly represses PME31 (pectin methylesterase 31).
GO:0048316 seed development	IMP PMID:10760247 The Arabidopsis abscisic acid response gene ABI5 encodes a b...	ACCEPT	Summary: ABI5 is predominantly seed-expressed, accumulates in the embryo during late seed maturation, and regulates LEA genes during seed development. Core developmental context. Reason: Supported by mutant analysis and expression during late embryogenesis; ABI5 controls ABA-dependent seed maturation gene expression. Supporting Evidence: PMID:10760247 ABI5 regulates a subset of late embryogenesis-abundant genes during both developmental stages file:ARATH/ABI5/ABI5-deep-research-falcon.md ABI5 as a master regulator of ABA-mediated growth arrest during the embryo-to-seedling transition
GO:0005634 nucleus	IDA PMID:15642716 Characterization of three homologous basic leucine zipper tr...	ACCEPT	Summary: Direct experimental evidence (GFP fusions) shows ABI5-family proteins localize constitutively to the nucleus, consistent with transcription factor function. Reason: Experimentally supported nuclear localization for a transcription factor. Supporting Evidence: PMID:15642716 the three fusion proteins were expressed with a largely overlapping pattern and constitutively localized in the nuclei file:ARATH/ABI5/ABI5-deep-research-falcon.md ABI5 acts in the nucleus. In native-promoter ABI5-GFP lines, ABI5 was predominantly nucleoplasmic after 4 h ABA treatment
GO:0009414 response to water deprivation	IEP PMID:11287670 A postgermination developmental arrest checkpoint is mediate...	KEEP AS NON CORE	Summary: ABI5 levels rise with imposed water stress and ABI5 protects germinated embryos from drought, acting within the drought/osmotic response. Peripheral to the core seed/ABA transcriptional role. Reason: Supported but a downstream stress-response role; ABI5's defining function is ABA-dependent transcriptional control. Supporting Evidence: PMID:11287670 ABI5 levels increase on imposition of water stress and decrease on stress removal
GO:0009651 response to salt stress	IEP PMID:11287670 A postgermination developmental arrest checkpoint is mediate...	KEEP AS NON CORE	Summary: ABI5 expression responds to salt exposure and salt cannot arrest growth in abi5 mutants, indicating ABI5 acts within the salt-stress response. Peripheral relative to the core function. Reason: Supported but downstream stress-response role; non-core. Supporting Evidence: PMID:11287670 ABI5 expression is regulated by drought and salt exposure
GO:0009737 response to abscisic acid	IEP PMID:11287670 A postgermination developmental arrest checkpoint is mediate...	ACCEPT	Summary: ABI5 accumulation, phosphorylation, stability, and activity are highly regulated by ABA; ABI5 mediates ABA repression of growth. Core ABA-response function (duplicate evidence code). Reason: Strongly supported core ABA-response role. Supporting Evidence: PMID:11287670 ABI5 protein accumulation, phosphorylation, stability, and activity are highly regulated by ABA during germination and early seedling growth
GO:0009737 response to abscisic acid	IMP PMID:11287670 A postgermination developmental arrest checkpoint is mediate...	ACCEPT	Summary: abi5 mutants show altered ABA sensitivity; ABI5 is necessary for ABA-mediated postgermination growth arrest. Core ABA-response role (IMP duplicate). Reason: Mutant evidence confirms ABI5 is required for the ABA response; core function. Supporting Evidence: PMID:11287670 ABI5 is necessary to maintain germinated embryos in a quiescent state thereby protecting plants from drought
GO:0010182 sugar mediated signaling pathway	TAS PMID:12663220 Sugar and hormone connections.	KEEP AS NON CORE	Summary: ABI5 is a component of ABA-sugar signaling crosstalk in seedling development; its expression is regulated by glucose. A peripheral role relative to the core seed/ABA transcriptional function. Reason: Supported by the sugar-hormone literature and glucose regulation of ABI5, but secondary to its core ABA-effector role. Supporting Evidence: PMID:12663220 Characterization of sugar-signalling mutants in Arabidopsis has unravelled a complex signalling network that links sugar responses to two plant stress hormones--abscisic acid and ethylene
GO:0003700 DNA-binding transcription factor activity	ISS PMID:11906833 bZIP transcription factors in Arabidopsis.	ACCEPT	Summary: ABI5 is classified as a group-A bZIP transcription factor; sequence similarity supports DNA-binding transcription factor activity. Duplicate of the core MF. Reason: Core molecular function, consistent with bZIP family classification and experimental data. Supporting Evidence: PMID:11906833 bZIP transcription factors in Arabidopsis
GO:0003700 DNA-binding transcription factor activity	ISS PMID:11118137 Arabidopsis transcription factors: genome-wide comparative a...	ACCEPT	Summary: ABI5 annotated as a transcription factor by genome-wide comparative analysis. Duplicate support for the core DNA-binding TF molecular function. Reason: Core molecular function, consistent with TF classification. Supporting Evidence: PMID:11118137 Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes
GO:0003677 DNA binding	IDA PMID:11489176 Physical interactions between ABA response loci of Arabidops...	MODIFY	Summary: Yeast one-hybrid shows ABI5 binds directly to the AtEm6 promoter fragment. Direct evidence of DNA binding. The more specific sequence-specific DNA binding / DNA-binding TF activity terms better capture the function. Reason: Generic DNA binding is supported but a transcription factor with defined ABRE specificity warrants the more informative term sequence-specific DNA binding (already annotated) and DNA-binding transcription factor activity. Proposed replacements: sequence-specific DNA binding Supporting Evidence: PMID:11489176 only ABI5 binds directly to this promoter fragment
GO:0003700 DNA-binding transcription factor activity	TAS PMID:10760247 The Arabidopsis abscisic acid response gene ABI5 encodes a b...	ACCEPT	Summary: ABI5 encodes a bZIP transcription factor whose DNA-binding and dimerization domains are required for function. Core MF (TAS duplicate). Reason: Core molecular function, directly supported by the cloning paper. Supporting Evidence: PMID:10760247 The previously characterized abi5-1 allele encodes a protein that lacks the DNA binding and dimerization domains required for ABI5 function.
GO:0003700 DNA-binding transcription factor activity	TAS PMID:11019812 A genomic perspective on plant transcription factors.	ACCEPT	Summary: ABI5 listed among plant transcription factors. Duplicate support for the core DNA-binding TF molecular function. Reason: Core molecular function. Supporting Evidence: PMID:11019812 A genomic perspective on plant transcription factors
GO:0006355 regulation of DNA-templated transcription	TAS PMID:11019812 A genomic perspective on plant transcription factors.	ACCEPT	Summary: ABI5 regulates transcription as a TF. Broad but correct process term (TAS duplicate). Reason: Accurate, if general, process annotation for a transcription factor. Supporting Evidence: PMID:11019812 A genomic perspective on plant transcription factors

Gene Description

Existing Annotations Review

Core Functions

Sequence-specific DNA-binding transcription factor that recognizes and binds the ABA-responsive element (ABRE) in the promoters of ABA- and seed maturation-regulated genes, acting as a transcriptional activator. Binds DNA as a homodimer or as heterodimers with related ABI5-subfamily bZIP factors.

Central transcriptional effector of abscisic acid signaling that activates late embryogenesis-abundant (LEA) and other ABA-responsive genes during seed maturation, and mediates ABA-dependent repression of seed germination and the postgermination developmental growth-arrest checkpoint.

References

Suggested Questions for Experts

Deep Research

Falcon

📚 Additional Documentation

Notes

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